Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation

Masaki Shibayama, Satona Ohno, Takashi Osaka, Reiko Sakamoto, Akinori Tokunaga, Yuhki Nakatake, Mitsuharu Sato, Nobuaki Yoshida

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)


Polypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splicing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site-dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear. Here, we report that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation. We also established Ptb-/- embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo. Furthermore, cell cycle analysis and a cell synchronization assay revealed that Ptb -/- ES cells have a prolonged G2/M phase. Thus, our data indicate that PTB is essential for early mouse development and ES cell proliferation.

Original languageEnglish
Pages (from-to)6658-6668
Number of pages11
JournalFEBS Journal
Issue number22
Publication statusPublished - 2009 Nov
Externally publishedYes


  • Cell cycle
  • Embryonic stem cells
  • Knockout mouse
  • Polypyrimidine tract-binding protein
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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