@article{0476448009de472e82604321fd066753,
title = "Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission",
abstract = "AMPA receptor (AMPAR) complexes contain auxiliary subunits that modulate receptor trafficking and gating. In addition to the transmembrane AMPAR regulatory proteins (TARPs) and cornichons (CNIH-2/3), recent proteomic studies identified a diverse array of additional AMPAR-associated transmembrane and secreted partners. We systematically surveyed these and found that PORCN and ABHD6 increase GluA1 levels in transfected cells. Knockdown of PORCN in rat hippocampal neurons, which express it in high amounts, selectively reduces levels of all tested AMPAR complex components. Regulation of AMPARs is independent of PORCN's membrane-associated O-acyl transferase activity. PORCN knockdown in hippocampal neurons decreases AMPAR currents and accelerates desensitization and leads to depletion of TARP γ-from AMPAR complexes.",
author = "Nadine Erlenhardt and Hong Yu and Kavitha Abiraman and Tokiwa Yamasaki and Wadiche, {Jacques I.} and Susumu Tomita and Bredt, {David S.}",
note = "Funding Information: This work is partially supported by NIH/NIMH MH085080 (S.T.) and NIH/NINDS NS065920 (J.I.W.). We thank the McMahon lab (UAB) for help and advice with the plasticity experiments. We thank Dr. Ignatia B. Van den Veyver (Baylor College of Medicine), Dr. Kevin R. Jones (University of Colorado-Boulder), and Jackson Laboratory for generating and maintaining conditional PORCN and Emx1(Cre) mice. Requests for PORCN KO mice should be addressed to susumu.tomita@yale.edu Funding Information: This work is partially supported by NIH/NIMH MH085080 (S.T.) and NIH/NINDS NS065920 (J.I.W.). We thank the McMahon lab (UAB) for help and advice with the plasticity experiments. We thank Dr. Ignatia B. Van den Veyver (Baylor College of Medicine), Dr. Kevin R. Jones (University of Colorado-Boulder), and Jackson Laboratory for generating and maintaining conditional PORCN and Emx1(Cre) mice. Requests for PORCN KO mice should be addressed to susumu.tomita@yale.edu Publisher Copyright: {\textcopyright} 2016 The Authors.",
year = "2016",
month = feb,
day = "2",
doi = "10.1016/j.celrep.2015.12.078",
language = "English",
volume = "14",
pages = "782--794",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "4",
}