Abstract
Ischemic preconditioning (PC) improves post-ischemic function, and heat shock (HS) mimics delayed PC in young animals. However, PC is not protective and the consequences of HS are not known in the aging hearts. This report examines the efficacy of HS and its synergy with PC in the middle-aged rat hearts. Hearts from 12- or 50-week-old rats were subjected to PC before 25min ischemia followed by 30min reperfusion 48h after HS. HS induced HS proteins (HSP) in both age groups but that PC and HS translocated PKC-α and -δ only in young rats. The beneficial effects of HS and PC were additive and enhanced protein kinase C (PKC) translocation in young rats. However, neither HS alone nor in combination with PC conferred any functional advantage or accelerated PKC translocation in old rats. Similarly neither HS alone nor in combination with PC restore PC effects in old rats with impaired PKC activation, despite the induction of HSP, indicating that induction of HSP is insufficient for cytoprotection.
| Original language | English |
|---|---|
| Pages (from-to) | 299-306 |
| Number of pages | 8 |
| Journal | Experimental Gerontology |
| Volume | 38 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2003 Mar 1 |
Keywords
- Aging
- Heart
- Heat shock
- Ischemia
- Ischemic preconditioning
- Protein kinase C
- Reperfusion
ASJC Scopus subject areas
- Biochemistry
- Ageing
- Molecular Biology
- Genetics
- Endocrinology
- Cell Biology