Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos

Ban Sato; Yohko U. Katagiri; Kenji Miyado; Hidenori Akutsu; Yoshitaka Miyagawa; Yasuomi Horiuchi; Hideki Nakajima; Hajime Okita; Akihiro Umezawa; Jun ichi Hata; Junichiro Fujimoto; Kiyotaka Toshimori; Nobutaka Kiyokawa

doi:10.1016/j.bbrc.2007.10.093

Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos

Ban Sato, Yohko U. Katagiri, Kenji Miyado, Hidenori Akutsu, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hideki Nakajima, Hajime Okita, Akihiro Umezawa, Jun ichi Hata, Junichiro Fujimoto, Kiyotaka Toshimori, Nobutaka Kiyokawa

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopentaosylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.

Original language	English
Pages (from-to)	838-843
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	364
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2007.10.093
Publication status	Published - 2007 Dec 28
Externally published	Yes

Keywords

Embryonal carcinoma cells
Embryonic stem cells
Immunostaining
Preimplantation embryo
SSEA-4
SialylGb5

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2007.10.093

Cite this

Sato, B., Katagiri, Y. U., Miyado, K., Akutsu, H., Miyagawa, Y., Horiuchi, Y., Nakajima, H., Okita, H., Umezawa, A., Hata, J. I., Fujimoto, J., Toshimori, K., & Kiyokawa, N. (2007). Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos. Biochemical and Biophysical Research Communications, 364(4), 838-843. https://doi.org/10.1016/j.bbrc.2007.10.093

Sato, B, Katagiri, YU, Miyado, K, Akutsu, H, Miyagawa, Y, Horiuchi, Y, Nakajima, H, Okita, H, Umezawa, A, Hata, JI, Fujimoto, J, Toshimori, K & Kiyokawa, N 2007, 'Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos', Biochemical and Biophysical Research Communications, vol. 364, no. 4, pp. 838-843. https://doi.org/10.1016/j.bbrc.2007.10.093

@article{b10dcfb08e1a4f89ab0ee3f98dfc9471,

title = "Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos",

abstract = "The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopentaosylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.",

keywords = "Embryonal carcinoma cells, Embryonic stem cells, Immunostaining, Preimplantation embryo, SSEA-4, SialylGb5",

author = "Ban Sato and Katagiri, {Yohko U.} and Kenji Miyado and Hidenori Akutsu and Yoshitaka Miyagawa and Yasuomi Horiuchi and Hideki Nakajima and Hajime Okita and Akihiro Umezawa and Hata, {Jun ichi} and Junichiro Fujimoto and Kiyotaka Toshimori and Nobutaka Kiyokawa",

note = "Funding Information: We thank S. Yamauchi for her excellent secretarial work. This work was supported in part by grants from CREST of JST and the 3rd. term comprehensive 10-year-strategy for cancer control, Research on Children and Families, Research on Human Genome Tailor made and Research on Publicly Essential Drugs and Medical Devices, Health and Labour Sciences Research Grants from the Ministry of Health, Labour and Welfare of Japan and a grant from The Japan Leukemia Research Field.",

year = "2007",

month = dec,

day = "28",

doi = "10.1016/j.bbrc.2007.10.093",

language = "English",

volume = "364",

pages = "838--843",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos

AU - Sato, Ban

AU - Katagiri, Yohko U.

AU - Miyado, Kenji

AU - Akutsu, Hidenori

AU - Miyagawa, Yoshitaka

AU - Horiuchi, Yasuomi

AU - Nakajima, Hideki

AU - Okita, Hajime

AU - Umezawa, Akihiro

AU - Hata, Jun ichi

AU - Fujimoto, Junichiro

AU - Toshimori, Kiyotaka

AU - Kiyokawa, Nobutaka

N1 - Funding Information: We thank S. Yamauchi for her excellent secretarial work. This work was supported in part by grants from CREST of JST and the 3rd. term comprehensive 10-year-strategy for cancer control, Research on Children and Families, Research on Human Genome Tailor made and Research on Publicly Essential Drugs and Medical Devices, Health and Labour Sciences Research Grants from the Ministry of Health, Labour and Welfare of Japan and a grant from The Japan Leukemia Research Field.

PY - 2007/12/28

Y1 - 2007/12/28

N2 - The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopentaosylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.

AB - The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopentaosylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.

KW - Embryonal carcinoma cells

KW - Embryonic stem cells

KW - Immunostaining

KW - Preimplantation embryo

KW - SSEA-4

KW - SialylGb5

UR - http://www.scopus.com/inward/record.url?scp=36049015104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36049015104&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.10.093

DO - 10.1016/j.bbrc.2007.10.093

M3 - Article

C2 - 18273440

AN - SCOPUS:36049015104

SN - 0006-291X

VL - 364

SP - 838

EP - 843

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this