Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y3Al5O 12:Ce3+ nanophosphor

Ikumi Kusayama; Ryo Asakura; Daisuke Saito; Tetsuhiko Isobe; Kiyoshi Kurokawa; Hideki Aizawa; Tomohiro Takagi; Yosuke Hirayama; Michio Ohkubo

Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y₃Al₅O ₁₂:Ce³⁺ nanophosphor

Ikumi Kusayama, Ryo Asakura, Daisuke Saito, Tetsuhiko Isobe, Kiyoshi Kurokawa, Hideki Aizawa, Tomohiro Takagi, Yosuke Hirayama, Michio Ohkubo

Department of Applied Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Positively charged Y₃Al₅O₁₂Ce³⁺ nanoparticles and negatively charged poly(sodium 4-styrenesulfonate) are sequentially adsorbed on the surface of negatively charged poly(methyl methacrylate) beads by the layer-by-layer adsorption method to obtain the composite beads, showing green emission under the blue light excitation. These fluorescent composite beads are detected as a dot group by flowcytometry (FCM). Bovine serum albumin (BSA) immomobilized on the composite beads with green emission are mixed with anti-BSA tagged with organic red dye. The antigen-antibody reaction is detected from both green and red emissions by FCM, showing that the fluorescent composite beads can be used for detecting biological reactions.

Original language	English
Title of host publication	ECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors
Pages	69-74
Number of pages	6
Edition	31
Publication status	Published - 2009
Event	Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting - Honolulu, HI, United States Duration: 2008 Oct 12 → 2008 Oct 17

Publication series

Name	ECS Transactions
Number	31
Volume	16
ISSN (Print)	1938-5862
ISSN (Electronic)	1938-6737

Other

Other	Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting
Country/Territory	United States
City	Honolulu, HI
Period	08/10/12 → 08/10/17

ASJC Scopus subject areas

Engineering(all)

Cite this

Kusayama, I., Asakura, R., Saito, D., Isobe, T., Kurokawa, K., Aizawa, H., Takagi, T., Hirayama, Y., & Ohkubo, M. (2009). Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y₃Al₅O ₁₂:Ce³⁺ nanophosphor. In ECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors (31 ed., pp. 69-74). (ECS Transactions; Vol. 16, No. 31).

Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y₃Al₅O ₁₂:Ce³⁺ nanophosphor. / Kusayama, Ikumi; Asakura, Ryo; Saito, Daisuke et al.
ECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors. 31. ed. 2009. p. 69-74 (ECS Transactions; Vol. 16, No. 31).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kusayama, I, Asakura, R, Saito, D, Isobe, T, Kurokawa, K, Aizawa, H, Takagi, T, Hirayama, Y & Ohkubo, M 2009, Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y₃Al₅O ₁₂:Ce³⁺ nanophosphor. in ECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors. 31 edn, ECS Transactions, no. 31, vol. 16, pp. 69-74, Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting, Honolulu, HI, United States, 08/10/12.

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abstract = "Positively charged Y3Al5O12Ce3+ nanoparticles and negatively charged poly(sodium 4-styrenesulfonate) are sequentially adsorbed on the surface of negatively charged poly(methyl methacrylate) beads by the layer-by-layer adsorption method to obtain the composite beads, showing green emission under the blue light excitation. These fluorescent composite beads are detected as a dot group by flowcytometry (FCM). Bovine serum albumin (BSA) immomobilized on the composite beads with green emission are mixed with anti-BSA tagged with organic red dye. The antigen-antibody reaction is detected from both green and red emissions by FCM, showing that the fluorescent composite beads can be used for detecting biological reactions.",

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AU - Isobe, Tetsuhiko

AU - Kurokawa, Kiyoshi

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AB - Positively charged Y3Al5O12Ce3+ nanoparticles and negatively charged poly(sodium 4-styrenesulfonate) are sequentially adsorbed on the surface of negatively charged poly(methyl methacrylate) beads by the layer-by-layer adsorption method to obtain the composite beads, showing green emission under the blue light excitation. These fluorescent composite beads are detected as a dot group by flowcytometry (FCM). Bovine serum albumin (BSA) immomobilized on the composite beads with green emission are mixed with anti-BSA tagged with organic red dye. The antigen-antibody reaction is detected from both green and red emissions by FCM, showing that the fluorescent composite beads can be used for detecting biological reactions.

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