Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography

H. Inoue; Y. Nonomura; A. Ashizawa; I. Tajima; M. Nomura; N. Nakamura; N. Yoshioka

doi:10.1007/BF02271011

Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography

H. Inoue, Y. Nonomura, A. Ashizawa, I. Tajima, M. Nomura, N. Nakamura, N. Yoshioka

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophyll a [Cu(II)-BCh]-a] and zinc(II) bacteriochlorophyll a [Zn(II)-BCh]-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25:75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

Original language	English
Pages (from-to)	361-364
Number of pages	4
Journal	Chromatographia
Volume	43
Issue number	7-8
DOIs	https://doi.org/10.1007/BF02271011
Publication status	Published - 1996

Keywords

Bacteriochlorophyll a
Column liquid chromatography
Copper(II) bacteriochlorophyll a
Zinc(II) bacteriochlorophyll a

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Organic Chemistry

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10.1007/BF02271011

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title = "Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography",

abstract = "Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophyll a [Cu(II)-BCh]-a] and zinc(II) bacteriochlorophyll a [Zn(II)-BCh]-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25:75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.",

keywords = "Bacteriochlorophyll a, Column liquid chromatography, Copper(II) bacteriochlorophyll a, Zinc(II) bacteriochlorophyll a",

author = "H. Inoue and Y. Nonomura and A. Ashizawa and I. Tajima and M. Nomura and N. Nakamura and N. Yoshioka",

year = "1996",

doi = "10.1007/BF02271011",

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volume = "43",

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journal = "Chromatographia",

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T1 - Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography

AU - Inoue, H.

AU - Nonomura, Y.

AU - Ashizawa, A.

AU - Tajima, I.

AU - Nomura, M.

AU - Nakamura, N.

AU - Yoshioka, N.

PY - 1996

Y1 - 1996

N2 - Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophyll a [Cu(II)-BCh]-a] and zinc(II) bacteriochlorophyll a [Zn(II)-BCh]-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25:75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

AB - Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophyll a [Cu(II)-BCh]-a] and zinc(II) bacteriochlorophyll a [Zn(II)-BCh]-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25:75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

KW - Bacteriochlorophyll a

KW - Column liquid chromatography

KW - Copper(II) bacteriochlorophyll a

KW - Zinc(II) bacteriochlorophyll a

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U2 - 10.1007/BF02271011

DO - 10.1007/BF02271011

M3 - Article

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SN - 0009-5893

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SP - 361

EP - 364

JO - Chromatographia

JF - Chromatographia

IS - 7-8

ER -

Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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