TY - JOUR
T1 - Preparation of a DNA Complex with Lipoglutamide Having Tetraethylene Glycol Tails, and Its Application to DNA Delivery into Tumor Cells1
AU - Sato, Toshinori
AU - Akino, Hiroyuki
AU - Okahata, Yoshio
PY - 1996/8
Y1 - 1996/8
N2 - We newly prepared a polyion complex of DNA with lipoglutamide, N,N′-bis(18-hydroxy-7,10,13,16-tetraoxaoctadecyl)-L-glutamide hydrochloride (abbreviated to 2EO4C6N+). Formation and characterization of the DNA/2EO4C6N+ complex were investigated by elemental analysis, melting temperature, and light-scattering measurements. Compaction of DNA by binding with the cationic 2EO4C6N+ was seen by multi-angle light scattering. Aggregation of the DNA/2EO4C6N+ complex was significantly depressed compared with the DNA complex without tetraethylene glycol. The DNA/2EO4C6N+ complex had low cytotoxicity and efficient internalization into tumor cells compared with native DNA.
AB - We newly prepared a polyion complex of DNA with lipoglutamide, N,N′-bis(18-hydroxy-7,10,13,16-tetraoxaoctadecyl)-L-glutamide hydrochloride (abbreviated to 2EO4C6N+). Formation and characterization of the DNA/2EO4C6N+ complex were investigated by elemental analysis, melting temperature, and light-scattering measurements. Compaction of DNA by binding with the cationic 2EO4C6N+ was seen by multi-angle light scattering. Aggregation of the DNA/2EO4C6N+ complex was significantly depressed compared with the DNA complex without tetraethylene glycol. The DNA/2EO4C6N+ complex had low cytotoxicity and efficient internalization into tumor cells compared with native DNA.
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U2 - 10.1246/bcsj.69.2335
DO - 10.1246/bcsj.69.2335
M3 - Article
AN - SCOPUS:0029809364
SN - 0009-2673
VL - 69
SP - 2335
EP - 2340
JO - Bulletin of the Chemical Society of Japan
JF - Bulletin of the Chemical Society of Japan
IS - 8
ER -