TY - JOUR
T1 - Preparation of conophylline affinity nano-beads and identification of a target protein
AU - Suzuki, Eriko
AU - Ogura, Hideki
AU - Kato, Kuniki
AU - Takei, Izumi
AU - Kabe, Yasuaki
AU - Handa, Hiroshi
AU - Umezawa, Kazuo
N1 - Funding Information:
This work was financially supported in part by grants from the programs Grants-in-Aid for Scientific Research on Priority Areas and Grants-in-Aid for the Global Centers of Excellence (GCOE) Program (2007–2012 to Keio University) of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT). This work was also supported by the High-Tech Research Center Project for Private Universities: matching fund subsidy from MEXT, 2006−2010.
PY - 2009/9/1
Y1 - 2009/9/1
N2 - Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing β-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of β-cells, and this probe should be useful to find other target proteins.
AB - Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing β-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of β-cells, and this probe should be useful to find other target proteins.
KW - ARL6IP
KW - Affinity-purification
KW - Amination
KW - Conophylline
KW - Nano-beads
UR - http://www.scopus.com/inward/record.url?scp=68649110935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68649110935&partnerID=8YFLogxK
U2 - 10.1016/j.bmc.2009.07.062
DO - 10.1016/j.bmc.2009.07.062
M3 - Article
C2 - 19679484
AN - SCOPUS:68649110935
SN - 0968-0896
VL - 17
SP - 6188
EP - 6195
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 17
ER -