TY - JOUR
T1 - Preparation of optically active 4-chlorophenylalanine from its racemate by deracemization technique using transformant Escherichia coli cells
AU - Kato, Dai Ichiro
AU - Miyamoto, Kenji
AU - Ohta, Hiromichi
N1 - Funding Information:
D.K. acknowledges to the financial support by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (JSPS Research Fellowships for Young Scientists). This study was performed through Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government.
PY - 2005/9
Y1 - 2005/9
N2 - We have developed a method for the preparation of L-4-chlorophenylalanine from its racemate with Escherichia coli cells expressing a single foreign gene. L-4-Chlorophenylalanine was obtained in a high optical yield by the inversion of configuration of its d-form via the tandem reactions catalyzed by d-amino acid dehydrogenase (DadA) and branched-chain amino acid aminotransferase (BCAAT). While we constructed a plasmid for BCAAT utilizing the gene from Sinorhizobium meliloti ATCC 51124, the first enzyme DadA was the dadA-gene product from E. coli host cell itself, which was activated by the addition of L-alanine in the growth medium.
AB - We have developed a method for the preparation of L-4-chlorophenylalanine from its racemate with Escherichia coli cells expressing a single foreign gene. L-4-Chlorophenylalanine was obtained in a high optical yield by the inversion of configuration of its d-form via the tandem reactions catalyzed by d-amino acid dehydrogenase (DadA) and branched-chain amino acid aminotransferase (BCAAT). While we constructed a plasmid for BCAAT utilizing the gene from Sinorhizobium meliloti ATCC 51124, the first enzyme DadA was the dadA-gene product from E. coli host cell itself, which was activated by the addition of L-alanine in the growth medium.
KW - Branched-chain amino acid aminotransferase gene
KW - DadA gene activation
KW - Microbial deracemization
KW - Preparation of L-4-chlorophenylalanine
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U2 - 10.1080/10242420500296295
DO - 10.1080/10242420500296295
M3 - Article
AN - SCOPUS:30844460323
SN - 1024-2422
VL - 23
SP - 375
EP - 379
JO - Biocatalysis and Biotransformation
JF - Biocatalysis and Biotransformation
IS - 5
ER -