Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

Yuji Kasuya; Keiji Fujimoto; Haruma Kawaguchi; Masaki Miyamoto; Takeo Juji; Akira Otaka; Susumu Funakoshi; Nobutaka Fujii

doi:10.1163/156856293X00050

Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

Yuji Kasuya, Keiji Fujimoto, Haruma Kawaguchi, Masaki Miyamoto, Takeo Juji, Akira Otaka, Susumu Funakoshi, Nobutaka Fujii

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

Original language	English
Pages (from-to)	369-380
Number of pages	12
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	4
Issue number	4
DOIs	https://doi.org/10.1163/156856293X00050
Publication status	Published - 1993

Keywords

Microsphere
RGDS
biospecific activity
cell adhesion
platelet aggregation

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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10.1163/156856293X00050

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title = "Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets",

abstract = "A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.",

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T1 - Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

AU - Kasuya, Yuji

AU - Fujimoto, Keiji

AU - Kawaguchi, Haruma

AU - Miyamoto, Masaki

AU - Juji, Takeo

AU - Otaka, Akira

AU - Funakoshi, Susumu

AU - Fujii, Nobutaka

PY - 1993

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N2 - A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

AB - A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

KW - Microsphere

KW - RGDS

KW - biospecific activity

KW - cell adhesion

KW - platelet aggregation

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Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

Abstract

Keywords

ASJC Scopus subject areas

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