Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells

Masahiko Hirokawa, Soichiro Miura, Takeharu Shigematsu, Hideo Yoshida, Ryota Hokari, Hajime Higuchi, Iwao Kurose, Hiroyuki Kimura, Hidetsugu Saito, Toshio Nakaki, Hiromasa Ishii

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.

Original languageEnglish
Pages (from-to)667-672
Number of pages6
JournalLife Sciences
Issue number7
Publication statusPublished - 1997 Jul 11
Externally publishedYes


  • Cell proliferation
  • Interleukin-2
  • Intestinal epithelial cells
  • Intraluminal pressure
  • Phospholipase C
  • Protein kinase C

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • Pharmacology, Toxicology and Pharmaceutics(all)


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