Presynaptic M1 muscarinic receptor modulates spontaneous release of acetylcholine from rat basal forebrain slices

Takeshi Suzuki; Kazuko Fujimoto; Hisayo Oohata; Koichiro Kawashima

doi:10.1016/0304-3940(88)90409-0

Presynaptic M₁ muscarinic receptor modulates spontaneous release of acetylcholine from rat basal forebrain slices

Takeshi Suzuki, Kazuko Fujimoto, Hisayo Oohata, Koichiro Kawashima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Spontaneous release of acetylcholine (ACh) from rat basal forebrain slices in the presence of cholinesterase inhibitor was directly determined using a specific radioimmunoassay for ACh. The release was calcium dependent. A consistent amount of ACh release was observed throughout the experiment. Atropine (10^-8 to 10^-5 M) and pirenzepine (10^-7 to 10^-5 M) enhanced spontaneous ACh release. These findings indicate the presence of an M₁ muscarinic autoreceptor that modulates spontaneous release of ACh in the rat basal forebrain.

Original language	English
Pages (from-to)	209-212
Number of pages	4
Journal	Neuroscience Letters
Volume	84
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(88)90409-0
Publication status	Published - 1988 Jan 22

Keywords

Acetylcholine release
Atropine
Autoreceptor
Basal forebrain
M muscarinic receptor
Pirenzepine
Radioimmunoassay

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0304-3940(88)90409-0

Cite this

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title = "Presynaptic M1 muscarinic receptor modulates spontaneous release of acetylcholine from rat basal forebrain slices",

abstract = "Spontaneous release of acetylcholine (ACh) from rat basal forebrain slices in the presence of cholinesterase inhibitor was directly determined using a specific radioimmunoassay for ACh. The release was calcium dependent. A consistent amount of ACh release was observed throughout the experiment. Atropine (10-8 to 10-5 M) and pirenzepine (10-7 to 10-5 M) enhanced spontaneous ACh release. These findings indicate the presence of an M1 muscarinic autoreceptor that modulates spontaneous release of ACh in the rat basal forebrain.",

keywords = "Acetylcholine release, Atropine, Autoreceptor, Basal forebrain, M muscarinic receptor, Pirenzepine, Radioimmunoassay",

author = "Takeshi Suzuki and Kazuko Fujimoto and Hisayo Oohata and Koichiro Kawashima",

note = "Funding Information: This work was supportedin part by a Grant from The Fujisawa Foundationfo r 1987.W e are gratefult o Ms. Misao Hisada, Ms. Tomoko Miyagawaa nd Ms. Akiko Nagoshi for their technicaal ssistance.",

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T1 - Presynaptic M1 muscarinic receptor modulates spontaneous release of acetylcholine from rat basal forebrain slices

AU - Suzuki, Takeshi

AU - Fujimoto, Kazuko

AU - Oohata, Hisayo

AU - Kawashima, Koichiro

N1 - Funding Information: This work was supportedin part by a Grant from The Fujisawa Foundationfo r 1987.W e are gratefult o Ms. Misao Hisada, Ms. Tomoko Miyagawaa nd Ms. Akiko Nagoshi for their technicaal ssistance.

PY - 1988/1/22

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N2 - Spontaneous release of acetylcholine (ACh) from rat basal forebrain slices in the presence of cholinesterase inhibitor was directly determined using a specific radioimmunoassay for ACh. The release was calcium dependent. A consistent amount of ACh release was observed throughout the experiment. Atropine (10-8 to 10-5 M) and pirenzepine (10-7 to 10-5 M) enhanced spontaneous ACh release. These findings indicate the presence of an M1 muscarinic autoreceptor that modulates spontaneous release of ACh in the rat basal forebrain.

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KW - Radioimmunoassay

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