TY - JOUR
T1 - Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters
AU - Hasuwa, Hidetoshi
AU - Iwasaki, Yuka W.
AU - Au Yeung, Wan Kin
AU - Ishino, Kyoko
AU - Masuda, Harumi
AU - Sasaki, Hiroyuki
AU - Siomi, Haruhiko
N1 - Funding Information:
We thank all members of the Siomi Laboratory, especially H. Ishizu and K. Murano, for discussions and comments on this work, I. Ishimatsu for preparing paraffin sections, and H. Nishihara (Tokyo Institute of Technology) for his comments on hamster TEs. We also thank J. Oishi of the Sasaki Laboratory and T. Akinaga (Laboratory for Research Support, Medical Institute of Bioregulation, Kyushu University) for their help with whole-genome bisulfite sequencing. We are grateful to P. Svoboda (IMG, Czech Academy of Sciences), J. Li (Model Animal Research Center, Nanjing Medical University) and L. Wu (Shanghai Institute of Biochemistry and cell Biology, CAS) for sharing unpublished data. We are also grateful to M. Okabe and M. Ikawa (Osaka University) for their comments on the manuscript. Funding included support from a Grant-in-Aid for the Japan Society for the Promotion of Science (JSPS) KAKENHI grant no. 20H03175 (to H.H.), the Takeda Science Foundation (to H.H.), a JSPS Fellows (18J22025) (to K.I.), from JSPS KAKENHI grant nos 19H05268 and 18H02421, and from JST PRESTO grant no. JPMJPR20E2 (to Y.W.I.), for Scientific Research (S) (25221003) and the Program of totipotency (19H05753) (to H. Siomi). H. Siomi is also a recipient of funding for the project for elucidating and controlling mechanisms of ageing and longevity (1005442) from the Japan Agency for Medical Research and Development (AMED). This work was also supported by JSPS KAKENHI grant no. JP18H05214 (H. Sasaki).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2021/9
Y1 - 2021/9
N2 - Many animals have a conserved adaptive genome defence system known as the Piwi-interacting RNA (piRNA) pathway, which is essential for germ cell development and function. Disruption of individual mouse Piwi genes results in male but not female sterility, leading to the assumption that PIWI genes play little or no role in mammalian oocytes. Here, we report the generation of PIWI-defective golden hamsters, which have defects in the production of functional oocytes. The mechanisms involved vary among the hamster PIWI genes, whereby the lack of PIWIL1 has a major impact on gene expression, including hamster-specific young transposon de-silencing, whereas PIWIL3 deficiency has little impact on gene expression in oocytes, although DNA methylation was reduced to some extent in PIWIL3-deficient oocytes. Our findings serve as the foundation for developing useful models to study the piRNA pathway in mammalian oocytes, including humans.
AB - Many animals have a conserved adaptive genome defence system known as the Piwi-interacting RNA (piRNA) pathway, which is essential for germ cell development and function. Disruption of individual mouse Piwi genes results in male but not female sterility, leading to the assumption that PIWI genes play little or no role in mammalian oocytes. Here, we report the generation of PIWI-defective golden hamsters, which have defects in the production of functional oocytes. The mechanisms involved vary among the hamster PIWI genes, whereby the lack of PIWIL1 has a major impact on gene expression, including hamster-specific young transposon de-silencing, whereas PIWIL3 deficiency has little impact on gene expression in oocytes, although DNA methylation was reduced to some extent in PIWIL3-deficient oocytes. Our findings serve as the foundation for developing useful models to study the piRNA pathway in mammalian oocytes, including humans.
UR - http://www.scopus.com/inward/record.url?scp=85114605449&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85114605449&partnerID=8YFLogxK
U2 - 10.1038/s41556-021-00745-3
DO - 10.1038/s41556-021-00745-3
M3 - Article
C2 - 34489571
AN - SCOPUS:85114605449
SN - 1465-7392
VL - 23
SP - 1002
EP - 1012
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 9
ER -