Proliferation of neural and neuronal progenitors after global brain ischemia in young adult macaque monkeys

Anton B. Tonchev, Tetsumori Yamashima, Liang Zhao, Hirotaka James Okano, Hideyuki Okano

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155 Citations (Scopus)


To investigate the effect of global cerebral ischemia on brain cell proliferation in young adult macaques, we infused 5-bromo-2′-deoxyuridine (BrdU), a DNA replication indicator, into monkeys subjected to ischemia or sham-operated. Subsequent quantification by BrdU immunohistochemistry revealed a significant postischemic increase in the number of BrdU-labeled cells in the hippocampal dentate gyrus, subventricular zone of the temporal horn of the lateral ventricle, and temporal neocortex. In all animals, 20-40% of the newly generated cells in the dentate gyrus and subventricular zone expressed the neural progenitor cell markers Musashi1 or Nestin. A few BrdU-positive cells in postischemic monkeys were double-stained for markers of neuronal progenitors (class III β-tubulin, TUC4, doublecortin, or Hu), neurons (NeuN), or glia (S100β or GFAP). Our results suggest that ischemia activates endogenous neuronal and glial precursors residing in diverse locations of the adult primate central nervous system.

Original languageEnglish
Pages (from-to)292-301
Number of pages10
JournalMolecular and Cellular Neuroscience
Issue number2
Publication statusPublished - 2003 Jun 1

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology


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