Protection of ischemie myocardium by inhibition of contracture in isolated rat heart

Masato Tani, Hiroshi Hasegawa, Yukako Suganuma, Ken Shinmura, Yoko Kayashi, Yoshiro Nakamura

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33 Citations (Scopus)

Abstract

Protection of the isch-emic myocardium by pretreatment with a high dose of 2,3-butanedione monoxime (: BDM is attributed to the enhancement of glycolytic ATP production rather than to the inhibition of contracture during mild ischemia. Our objective was to investigate whether the inhibition of contracture would protect the arrested heart during prolonged ischemia. Isolated perfused rat hearts were subjected to 30 min of low-flow ischemia followed by reperfusion. Ischémie hearts were treated with BDM 5 mmol/1 after beating stopped. BDM ameliorated the increase in intraventricular pressure after ischemia without significant changes in ATP levels and with a decreased accumulation of lactate. BDM treatment accelerated the recovery of function and high-energy phosphates with reduced myocardial Ca2+ overload. The results of this study suggested that inhibition of contracture can protect the heart from ischemia-reperfusion injury.

Original languageEnglish
Pages (from-to)H2515-H2519
JournalAmerican Journal of Physiology
Volume271
Issue number6 PART 2
Publication statusPublished - 1996

Keywords

  • 2,3-butanedione monoxime
  • Energy-sparing effect
  • Negative inotropic effect

ASJC Scopus subject areas

  • Physiology (medical)

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