@inproceedings{a70ff662687b43d69eb31093ff23c2da,
title = "Protein Expression Micro-Scale Mapping Analysis of Endothelial Tissue in in Vitro Branched Vascular Model under Mechanical Stimuli",
abstract = "This paper describes a micro-scale mapping of protein expression on endothelial tissue in an extracellular matrix (ECM)-based branched microchannel under mechanical stimuli. Our system mimics the heterogeneity in the distribution of complex mechanical stimuli applied to tissues in vivo by applying mechanical stimuli to a branched vascular model. We confirmed the up-regulation of gene expression levels in vascular endothelial cells(ECs) by stretch stress. We cultured ECs under complex mechanical stimuli, and visualized the distribution of mechanotransducer protein expression in the branched vascular model. We believe that our system could open a new door for the analysis of local cellular responses to mechanical stimuli.",
keywords = "Endothelial cells, Immunostaining, Microchannel, Tissue engineering",
author = "Jumpei Muramatsu and Azusa Shimizu and Michinao Hashimoto and Shigenori Miura and Hiroaki Onoe",
note = "Funding Information: This work was partly supported by Grant-in Aid for Scientific Research (B) (19H04440) from Japan Society for the Promotion of Science (JSPS), Japan. M.H. acknowledges the Ministry of Education (MOE), Singapore, for the project funding (MOE2019-T2-2-192). Publisher Copyright: {\textcopyright} 2022 IEEE.; 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 ; Conference date: 09-01-2022 Through 13-01-2022",
year = "2022",
doi = "10.1109/MEMS51670.2022.9699768",
language = "English",
series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "283--286",
booktitle = "35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022",
}