TY - JOUR
T1 - Protein kinase C δ and η differently regulate the expression of loricrin and Jun family proteins in human keratinocytes
AU - Kamioka, Nagisa
AU - Akahane, Tomoko
AU - Kohno, Yoko
AU - Kuroki, Toshio
AU - Iijima, Masafumi
AU - Honma, Ikuo
AU - Ohba, Motoi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan ( 19590107 ). We thank Andrew Boerger and Noriko Shishido for constructive comments and corrections of the English of this work.
PY - 2010/3/26
Y1 - 2010/3/26
N2 - Barrier function of the epidermis is maintained by precise expression of keratinocyte-specific structural proteins to form the cornified cell envelope (CE). Loricrin, a major component of the CE, is expressed at the late stage of keratinocyte differentiation. In this study, we reveal the isoform-specific function of protein kinase C (PKC) in the regulation of loricrin expression. Both PKCδ and PKCη have been recognized as differentiation-promoting isoforms. However, loricrin expression was inversely controlled by PKCδ and PKCη in cultured keratinocytes and 3D skin culture; i.e. loricrin expression was decreased by PKCδ and increased by PKCη. To clarify the mechanisms that PKCδ and PKCη oppositely regulate the loricrin expression, we examined the expression of activator protein-1 (AP-1) family proteins, which modulate the transcription of loricrin and are downstream molecules of PKC. PKCδ decreased c-Jun expression, whereas PKCη increased JunD, which are positive regulators of loricrin transcription. These findings suggest that inverse effects of PKCδ and PKCη on loricrin expression attributes to the expression of c-Jun and JunD.
AB - Barrier function of the epidermis is maintained by precise expression of keratinocyte-specific structural proteins to form the cornified cell envelope (CE). Loricrin, a major component of the CE, is expressed at the late stage of keratinocyte differentiation. In this study, we reveal the isoform-specific function of protein kinase C (PKC) in the regulation of loricrin expression. Both PKCδ and PKCη have been recognized as differentiation-promoting isoforms. However, loricrin expression was inversely controlled by PKCδ and PKCη in cultured keratinocytes and 3D skin culture; i.e. loricrin expression was decreased by PKCδ and increased by PKCη. To clarify the mechanisms that PKCδ and PKCη oppositely regulate the loricrin expression, we examined the expression of activator protein-1 (AP-1) family proteins, which modulate the transcription of loricrin and are downstream molecules of PKC. PKCδ decreased c-Jun expression, whereas PKCη increased JunD, which are positive regulators of loricrin transcription. These findings suggest that inverse effects of PKCδ and PKCη on loricrin expression attributes to the expression of c-Jun and JunD.
KW - Differentiation
KW - Keratinocytes
KW - Protein kinase C
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U2 - 10.1016/j.bbrc.2010.02.125
DO - 10.1016/j.bbrc.2010.02.125
M3 - Article
C2 - 20184865
AN - SCOPUS:77949873009
SN - 0006-291X
VL - 394
SP - 106
EP - 111
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -