TY - JOUR
T1 - Proteomic analysis of plasma proteins in Japanese semisuper centenarians
AU - Miura, Yuri
AU - Sato, Yuji
AU - Arai, Yasumichi
AU - Abe, Yukiko
AU - Takayama, Michiyo
AU - Toda, Tosifusa
AU - Hirose, Nobuyoshi
AU - Endo, Tamao
N1 - Funding Information:
We dedicate this manuscript to the life and career of Yuji Sato, who died on June 21, 2006. We thank Dr. Yoko Sugihara (TMIG) for her helpful advice on statistical analysis, and Shimadzu Biotech and Bio-Rad Laboratories for technical assistance with MALDI-TOF/MS and the analyses of gel images, respectively. We also thank Drs. Yasuyuki Gondo and Hiroki Inagaki for helpful discussions. This study was partly supported by a Grant-in-Aid for Scientific Research (B) (No. 20390031 to T.E.) from the Japan Society for the Promotion of Science, and partly by Mitsui Sumitomo Insurance Welfare Foundation (to Y.M.).
PY - 2011/1
Y1 - 2011/1
N2 - We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (>105years) and young controls (20-39years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin β-chain, α1-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, α1-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs.
AB - We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (>105years) and young controls (20-39years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin β-chain, α1-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, α1-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs.
KW - Aging
KW - Oxidative stress
KW - Plasma proteins
KW - Proteomics
KW - Semisuper centenarian
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U2 - 10.1016/j.exger.2010.10.002
DO - 10.1016/j.exger.2010.10.002
M3 - Article
C2 - 20951194
AN - SCOPUS:78649864568
SN - 0531-5565
VL - 46
SP - 81
EP - 85
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 1
ER -