Proto-oncogene Expression in Three Human Hepatoma Cell Lines, HCC-M, HCC-T and PLC/PRF/5

Hidetsugu Saito, Tatehiro Kagawa, Shingo Miyaguchi, Masaharu Tsuchiya, Toshio Morizane, Tetsu Watanabe, Naoki Kumagai, Kanji Tsuchimoto

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10 Citations (Scopus)


Changes of nucleotide sequences and expressions of cellular oncogenes in human hepatoma cell lines, PLC/PRF/5, HCC-M and HCC-T cells, were examined by Southern and Northern blot analyses. The probes used are DNA fragment of myc, N-, H-, K-ras, fos, fms, raf, erb-A, erb-B, and erb-B2 genes and synthetic oligonucleotides corresponding to the part of N-, H-, K-ras genes. The results are as follows. DNA amplification and rearrangement were not detected in these three human hepatoma cell lines. Point mutations at codons 12, 13, and 61 in N- and K-ras genes were not demonstrated in these cell lines. N-, H-, K-ras and myc transcripts were detected in these three cell lines. However, fos gene transcript was detected only in PLC/PRF/5 and HCC-M cells which were derived from hepatitis B related hepatocellular carcinoma and having integrated hepatitis B virus (HBV) DNA. These data showed that there are no specific proto-oncogene expression into RNA except for myc and ras genes, nor DNA rearrangement in these 3 human hepatoma cell lines with regards to at least 10 different oncogenes examined and suggest the relationship between fos gene expression and integration of HBV DNA in host cell DNA.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
Journalthe keio journal of medicine
Issue number3
Publication statusPublished - 1991


  • DNA
  • RNA
  • hepatocellular carcinoma
  • point mutation
  • proto-oncogene

ASJC Scopus subject areas

  • General Medicine


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