Protocol to isolate and enrich mouse splenic naive CD4+ T cells for in vitro CD4+CD8αα+ cell induction

Yosuke Harada, Kentaro Miyamoto, Tomohisa Sujino

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Recent studies have shown that CD4+CD8αα+ T cells are induced in the hypoxic environment of the small intestinal epithelium. Herein, we describe a protocol for CD4+CD8αα+ T cell induction from freshly isolated naive CD4+ T cells, including procedures for the isolation and enrichment of mouse splenic T cells. In addition, we present an approach that can induce more CD4+CD8αα+ T cells by artificially creating a hypoxic environment in vitro. For complete details on the use and execution of this protocol, please refer to Harada et al. (2022).

Original languageEnglish
Article number101728
JournalSTAR Protocols
Volume3
Issue number4
DOIs
Publication statusPublished - 2022 Dec 16

Keywords

  • Cell biology
  • Cell culture
  • Cell isolation
  • Flow cytometry/Mass cytometry
  • Immunology

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology

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