Pulmonary dysfunction in neonatal SP-B-deficient mice

Keisuke Tokieda, Jeffrey A. Whitsett, Jean C. Clark, Timothy E. Weaver, Kazushige Ikeda, Keith B. McConnell, Alan H. Jobe, Machiko Ikegami, Harriet S. Iwamoto

Research output: Contribution to journalArticlepeer-review

118 Citations (Scopus)


Pulmonary function was assessed in newborn wild-type and homozygous and heterozygous surfactant protein B (SP-B)-deficient mice after birth. SP-B +/+ and SP-B+/- mice became well oxygenated and survived postnatally. Although lung compliance was decreased slightly in the SP-B+/- mice, lung volumes and compliances were decreased markedly in homozygous SP-B-/- mice. They died rapidly after birth, failing to inflate their lungs or oxygenate. SP-B proprotein was absent in the SP-B-/- mice and was reduced in the SP-B+/- mice, as assessed by Western analysis. Surfactant protein A, surfactant proprotein C, surfactant protein D, and surfactant phospholipid content in lungs from SP-B+/- and SP-B-/- mice were not altered. Lung saturated phosphatidylcholine and precursor incorporation into saturated phosphatidylcholine were not influenced by SP-B genotype. Intratracheal administration of perfluorocarbon resulted in lung expansion, oxygenation, and prolonged survival of SP-B-/- mice and in reduced lung compliance in SP- B+/+ and SP-B+/- mice. Lack of SP-B caused respiratory failure at birth, and decreased SP-B protein was associated with reduced lung compliance. These findings demonstrate the critical role of SP-B in perinatal adaptation to air breathing.

Original languageEnglish
Pages (from-to)L875-L882
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number4 17-4
Publication statusPublished - 1997
Externally publishedYes


  • Lung compliance
  • Lung function
  • Perfluorocarbon
  • Respiratory distress syndrome
  • Surfactant protein

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


Dive into the research topics of 'Pulmonary dysfunction in neonatal SP-B-deficient mice'. Together they form a unique fingerprint.

Cite this