PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series

Yuji Kouzaki; Kei Mikita; Takuya Maeda; Masashi Ishihara; Shuichi Kawano; Yuji Fujikura; Kazuo Imai; Soichiro Kanoh; Kazuho Utsunomiya; Masahiro Inoue; Yasushi Miyahira; Akihiko Kawana

doi:10.2169/internalmedicine.54.4615

PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series

Yuji Kouzaki, Kei Mikita, Takuya Maeda, Masashi Ishihara, Shuichi Kawano, Yuji Fujikura, Kazuo Imai, Soichiro Kanoh, Kazuho Utsunomiya, Masahiro Inoue, Yasushi Miyahira, Akihiko Kawana

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.

Original language	English
Pages (from-to)	1447-1450
Number of pages	4
Journal	Internal Medicine
Volume	54
Issue number	11
DOIs	https://doi.org/10.2169/internalmedicine.54.4615
Publication status	Published - 2015 Jun 1
Externally published	Yes

Keywords

Diagnosis
Extrapulmonary tuberculosis
Loop-mediated isothermal amplification
Mycobacterium tuberculosis
Procedure for ultra-rapid extraction

ASJC Scopus subject areas

Internal Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2169/internalmedicine.54.4615

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title = "PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series",

abstract = "Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.",

keywords = "Diagnosis, Extrapulmonary tuberculosis, Loop-mediated isothermal amplification, Mycobacterium tuberculosis, Procedure for ultra-rapid extraction",

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doi = "10.2169/internalmedicine.54.4615",

language = "English",

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T1 - PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis

T2 - A case series

AU - Kouzaki, Yuji

AU - Mikita, Kei

AU - Maeda, Takuya

AU - Ishihara, Masashi

AU - Kawano, Shuichi

AU - Fujikura, Yuji

AU - Imai, Kazuo

AU - Kanoh, Soichiro

AU - Utsunomiya, Kazuho

AU - Inoue, Masahiro

AU - Miyahira, Yasushi

AU - Kawana, Akihiko

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.

AB - Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.

KW - Diagnosis

KW - Extrapulmonary tuberculosis

KW - Loop-mediated isothermal amplification

KW - Mycobacterium tuberculosis

KW - Procedure for ultra-rapid extraction

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JO - Internal Medicine

JF - Internal Medicine

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ER -

PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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