TY - JOUR
T1 - Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system
AU - Kiryo, Tetsushi
AU - Takahashi, Yuuwa
AU - Miyata, Shogo
N1 - Funding Information:
This research was partially supported by a research grant (Adaptable and Seamless Technology Transfer Program through Target‐Driven R&D) from JST. The authors are grateful to Shinya Takeuchi of Nissha Co., Ltd. for fabricating the micro transparent electrodes and the PDMS microfluidic chamber.
Funding Information:
This research was partially supported by a research grant (Adaptable and Seamless Technology Transfer Program through Target-Driven R&D) from JST. The authors are grateful to Shinya Takeuchi of Nissha Co., Ltd. for fabricating the micro transparent electrodes and the PDMS microfluidic chamber.
Publisher Copyright:
© 2022 The Authors. Engineering in Life Sciences published by Wiley-VCH GmbH.
PY - 2022/5
Y1 - 2022/5
N2 - Pluripotent stem cells (PSCs) such as embryonic stem cells and induced PSCs can differentiate into all somatic cell types such as cardiomyocytes, nerve cells, and chondrocytes. However, PSCs can easily lose their pluripotency if the culture process is disturbed. Therefore, cell sorting methods for purifying PSCs with pluripotency are important for the establishment and expansion of PSCs. In this study, we focused on dielectrophoresis (DEP) to separate cells without fluorescent dyes or magnetic antibodies. The goal of this study was to establish a cell sorting method for the purification of PSCs based on their pluripotency using DEP and a flow control system. The dielectrophoretic properties of mouse embryonic stem cells (mESCs) with and without pluripotency were evaluated in detail, and mESCs exhibited varying frequency dependencies in the DEP response. Based on the variance in DEP properties, mixed cell suspensions of mESCs can be separated according to their pluripotency with an efficacy of approximately 90%.
AB - Pluripotent stem cells (PSCs) such as embryonic stem cells and induced PSCs can differentiate into all somatic cell types such as cardiomyocytes, nerve cells, and chondrocytes. However, PSCs can easily lose their pluripotency if the culture process is disturbed. Therefore, cell sorting methods for purifying PSCs with pluripotency are important for the establishment and expansion of PSCs. In this study, we focused on dielectrophoresis (DEP) to separate cells without fluorescent dyes or magnetic antibodies. The goal of this study was to establish a cell sorting method for the purification of PSCs based on their pluripotency using DEP and a flow control system. The dielectrophoretic properties of mouse embryonic stem cells (mESCs) with and without pluripotency were evaluated in detail, and mESCs exhibited varying frequency dependencies in the DEP response. Based on the variance in DEP properties, mixed cell suspensions of mESCs can be separated according to their pluripotency with an efficacy of approximately 90%.
KW - cell sorting
KW - dielectrophoresis
KW - embryonic stem cell
KW - pluripotency
KW - pluripotent stem cell
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U2 - 10.1002/elsc.202100113
DO - 10.1002/elsc.202100113
M3 - Article
AN - SCOPUS:85128032224
SN - 1618-0240
VL - 22
SP - 417
EP - 426
JO - Engineering in Life Sciences
JF - Engineering in Life Sciences
IS - 5
ER -