TY - JOUR
T1 - Purification of two transcription factors required for initiation by mammalian RNA polymerase II
AU - Kawaguchi, Tatsuo
AU - Kitajima, Shigetaka
AU - Niho, Yoshiyuki
AU - Oda, Taiko
AU - Germino, Joseph
AU - Weissman, Sherman M.
AU - Yasukochi, Yukio
PY - 1990/9
Y1 - 1990/9
N2 - We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of ≈33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.
AB - We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of ≈33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.
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U2 - 10.1073/pnas.87.17.6619
DO - 10.1073/pnas.87.17.6619
M3 - Article
C2 - 2395865
AN - SCOPUS:0025059447
SN - 0027-8424
VL - 87
SP - 6619
EP - 6623
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -