Purified opioid μ-receptor is of a different molecular size than δ- and κ-receptors

Hiroshi Ueda, Hitoshi Harada, Hidemi Misawa, Masakatsu Nozaki, Hiroshi Takagi

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Opioid μ-receptors were solubilized from rat brains with 0.5% Triton X-100 in the presence of 0.32 M sucrose and then purified with 6-succinyl morphine-Affi-Gel 102 column. Purified materials showed a major band with mol.wt. 58,000 Da and a minor band with mol.wt. 41,000 Da. Affinity cross linking with [3H]DAGO, a selective μ-opioid agonist, to the neural membranes and purified materials revealed that opioid μ-binding protein has a mol.wt. of 58,000 Da, a value totally different from that of δ- (mol.wt. 18,500 Da) and κ- (mol.wt. 36,600 Da) binding protein.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalNeuroscience Letters
Issue number3
Publication statusPublished - 1987 Apr 10
Externally publishedYes


  • 6-Succinyl morphine-Affi-Gel 102
  • Cross linking
  • Opioid μ-receptor
  • Rat brain

ASJC Scopus subject areas

  • General Neuroscience


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