TY - JOUR
T1 - Pyrrolidinium-type fullerene derivative-induced apoptosis by the generation of reactive oxygen species in HL-60 cells
AU - Nishizawa, Chiho
AU - Hashimoto, Noriyuki
AU - Yokoo, Sachiko
AU - Funakoshi-Tago, Megumi
AU - Kasahara, Tadashi
AU - Takahashi, Kyoko
AU - Nakamura, Shigeo
AU - Mashino, Tadahiko
PY - 2009
Y1 - 2009
N2 - The biological activities of C60-bis(N,N-dimethylpyrrolidinium iodide), a water-soluble cationic fullerene derivative, on human promyeloleukaemia (HL-60) cells were investigated. The pyrrolidinium fullerene derivative showed cytotoxicity in HL-60 cells. The characteristics of apoptosis, such as DNA fragmentation and condensation of chromatin in HL-60 cells, were observed by exposure to the pyrrolidinium fullerene derivative. Caspase-3 and-8 were activated and cytochrome c was also released from mitochondria. The generation of reactive oxygen species (ROS) by the pyrrolidinium fullerene derivative was observed by DCFH-DA, a fluorescence probe for the detection of ROS. Pre-treatment with α-tocopherol suppressed cell death and intracellular oxidative stress caused by the pyrrolidinium fullerene derivative. The apoptotic cell death induced by the pyrrolidinium fullerene derivative was suggested to be mediated by ROS generated by the pyrrolidinium fullerene derivative.
AB - The biological activities of C60-bis(N,N-dimethylpyrrolidinium iodide), a water-soluble cationic fullerene derivative, on human promyeloleukaemia (HL-60) cells were investigated. The pyrrolidinium fullerene derivative showed cytotoxicity in HL-60 cells. The characteristics of apoptosis, such as DNA fragmentation and condensation of chromatin in HL-60 cells, were observed by exposure to the pyrrolidinium fullerene derivative. Caspase-3 and-8 were activated and cytochrome c was also released from mitochondria. The generation of reactive oxygen species (ROS) by the pyrrolidinium fullerene derivative was observed by DCFH-DA, a fluorescence probe for the detection of ROS. Pre-treatment with α-tocopherol suppressed cell death and intracellular oxidative stress caused by the pyrrolidinium fullerene derivative. The apoptotic cell death induced by the pyrrolidinium fullerene derivative was suggested to be mediated by ROS generated by the pyrrolidinium fullerene derivative.
KW - Apoptosis
KW - Caspase
KW - Fullerene derivative
KW - HL-60
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=70449645507&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449645507&partnerID=8YFLogxK
U2 - 10.3109/10715760903273849
DO - 10.3109/10715760903273849
M3 - Article
C2 - 19905986
AN - SCOPUS:70449645507
SN - 1071-5762
VL - 43
SP - 1240
EP - 1247
JO - Free Radical Research
JF - Free Radical Research
IS - 12
ER -