@article{f85b99c26c3a41eb8f5f756cdb461f84,
title = "Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device",
abstract = "Proper determination of the cell division axis is essential during development. Wnt3a is a known regulator of the cell division axis; however, the sensitivity of cells to Wnt3a signalling and its role in determining the cell division axis have not been measured to date. To address this gap, we took advantage of the asymmetric distribution of outer dense fibre 2 (ODF2/cenexin) proteins on centrosomes in dividing cells. To precisely quantify the sensitivity of cells to Wnt3a signalling, we developed a microfluidic cell culture device, which can produce a quantitative gradient of signalling molecules. We confirmed that mitotic SH-SY5Y neuroblastoma cells could detect a 2.5 ~ 5 × 10−3 nm·μm−1 Wnt3a concentration gradient and demonstrated that this gradient is sufficient to affect the determination of the pole-to-pole axis of cell division during the later stages of mitosis.",
keywords = "Wnt3a, microfluidics, neuroblastoma, pole-to-pole axis, quantitative gradient ODF2",
author = "Takumi Hiraiwa and Yuichiro Nakai and Yamada, {Takahiro G.} and Ryuichi Tanimoto and Hiroshi Kimura and Yoshinori Matsumoto and Norihisa Miki and Noriko Hiroi and Akira Funahashi",
note = "Funding Information: We thank Mr. Kaito Ii (Keio University, Japan), Ms. Mandy Engelsma (Handfast Point, The Netherlands) and Ozy Abram (Uni-edit Japan, Hong Kong) for their critical advice on English usage and proofreading of the manuscript. We also thank Dr. Hiroki Ota (Yokohama National University, Japan) and Dr. Tau-Mu Yi (University of California, Irvine, USA) for their crucial advice on the technical terms and structuring of the Materials and methods section. We are grateful to Dr. Viji Draviam-Sastry (Queen Mary University of London, UK) for giving essential advice on improving our manuscript from a biological point of view. We are deeply grateful to Prof. Hiroaki Kitano for his kind helps to establish this paper. This work was supported by the Japan Society for the Promotion of Science (JSPS), a Grant-in-Aid for Scientific Research (Kiban C) 15K06925 awarded to NH and a Grant-in-Aid for Challenging Exploratory Research (Chousenteki Houga) 26640132, awarded to AF. The funders had no role in the study design, data collection, analysis, decision to publish or preparation of the manuscript. Funding Information: We thank Mr. Kaito Ii (Keio University, Japan), Ms. Mandy Engelsma (Handfast Point, The Netherlands) and Ozy Abram (Uni-edit Japan, Hong Kong) for their critical advice on English usage and proofreading of the manuscript. We also thank Dr. Hiroki Ota (Yokohama National University, Japan) and Dr. Tau-Mu Yi (University of California, Irvine, USA) for their crucial advice on the technical terms and structuring of the materials and Methods section. We are grateful to Dr. Viji Draviam-Sastry (Queen Mary University of London, UK) for giving essential advice on improving our manuscript from a biological point of view. We are deeply grateful to Prof. Hiroaki Kitano for his kind helps to establish this paper. This work was supported by the Japan Society for the Promotion of Science (JSPS), a Grant-in-Aid for Scientific Research (Kiban C) 15K06925 awarded to NH and a Grant-in-Aid for Challenging Exploratory Research (Chousenteki Houga) 26640132, awarded to AF. The funders had no role in the study design, data collection, analysis, decision to publish or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.",
year = "2018",
month = dec,
doi = "10.1002/2211-5463.12525",
language = "English",
volume = "8",
pages = "1920--1935",
journal = "FEBS Open Bio",
issn = "2211-5463",
publisher = "Elsevier BV",
number = "12",
}