TY - JOUR
T1 - Quantitative evaluation of analyte transport on microfluidic paper-based analytical devices (μPADs)
AU - Ota, Riki
AU - Yamada, Kentaro
AU - Suzuki, Koji
AU - Citterio, Daniel
N1 - Funding Information:
K. Y. kindly acknowledges the funding from a Research Fellowship of the Japan Society for the Promotion of Science (JSPS) for Young Scientists.
Publisher Copyright:
© 2018 The Royal Society of Chemistry.
PY - 2018/2/7
Y1 - 2018/2/7
N2 - The transport efficiency during capillary flow-driven sample transport on microfluidic paper-based analytical devices (μPADs) made from filter paper has been investigated for a selection of model analytes (Ni2+, Zn2+, Cu2+, PO43-, bovine serum albumin, sulforhodamine B, amaranth) representing metal cations, complex anions, proteins and anionic molecules. For the first time, the transport of the analytical target compounds rather than the sample liquid, has been quantitatively evaluated by means of colorimetry and absorption spectrometry-based methods. The experiments have revealed that small paperfluidic channel dimensions, additional user operation steps (e.g. control of sample volume, sample dilution, washing step) as well as the introduction of sample liquid wicking areas allow to increase analyte transport efficiency. It is also shown that the interaction of analytes with the negatively charged cellulosic paper substrate surface is strongly influenced by the physico-chemical properties of the model analyte and can in some cases (Cu2+) result in nearly complete analyte depletion during sample transport. The quantitative information gained through these experiments is expected to contribute to the development of more sensitive μPADs.
AB - The transport efficiency during capillary flow-driven sample transport on microfluidic paper-based analytical devices (μPADs) made from filter paper has been investigated for a selection of model analytes (Ni2+, Zn2+, Cu2+, PO43-, bovine serum albumin, sulforhodamine B, amaranth) representing metal cations, complex anions, proteins and anionic molecules. For the first time, the transport of the analytical target compounds rather than the sample liquid, has been quantitatively evaluated by means of colorimetry and absorption spectrometry-based methods. The experiments have revealed that small paperfluidic channel dimensions, additional user operation steps (e.g. control of sample volume, sample dilution, washing step) as well as the introduction of sample liquid wicking areas allow to increase analyte transport efficiency. It is also shown that the interaction of analytes with the negatively charged cellulosic paper substrate surface is strongly influenced by the physico-chemical properties of the model analyte and can in some cases (Cu2+) result in nearly complete analyte depletion during sample transport. The quantitative information gained through these experiments is expected to contribute to the development of more sensitive μPADs.
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U2 - 10.1039/c7an01702b
DO - 10.1039/c7an01702b
M3 - Article
C2 - 29185559
AN - SCOPUS:85041070963
SN - 0003-2654
VL - 143
SP - 643
EP - 653
JO - Analyst
JF - Analyst
IS - 3
ER -