@article{6dd1f10a54144d11ba25150578f6f0c7,
title = "Rab21 regulates caveolin-1-mediated endocytic trafficking to promote immature neurite pruning",
abstract = "Transmembrane proteins are internalized by clathrin- and caveolin-dependent endocytosis. Both pathways converge on early endosomes and are thought to share the small GTPase Rab5 as common regulator. In contrast to this notion, we show here that the clathrin- and caveolin-mediated endocytic pathways are differentially regulated. Rab5 and Rab21 localize to distinct populations of early endosomes in cortical neurons and preferentially regulate clathrin- and caveolin-mediated pathways, respectively, suggesting heterogeneity in the early endosomes, rather than a converging point. Suppression of Rab21, but not Rab5, results in decreased plasma membrane localization and total protein levels of caveolin-1, which perturbs immature neurite pruning of cortical neurons, an in vivo-specific step of neuronal maturation. Taken together, our data indicate that clathrin- and caveolin-mediated endocytic pathways run in parallel in early endosomes, which show different molecular regulation and physiological function.",
keywords = "Rab21, caveolin-mediated endocytosis, early endosomes, neuronal maturation, neuronal migration",
author = "Mima Shikanai and Shiho Ito and Nishimura, {Yoshiaki V.} and Remi Akagawa and Mitsunori Fukuda and Michisuke Yuzaki and Nabeshima, {Yo ichi} and Takeshi Kawauchi",
note = "Funding Information: We thank R.L. Huganir, B.J. Knoll, and D.L. Turner for providing plasmids, and R.T. Yu for proofreading of the manuscript. We also thank the Core Instrumentation Facility, Keio University School of Medicine for help with Leica SP5 confocal microscopy. This work was supported by JSPS KAKENHI Grant Numbers JP26290015 and JP21H02655 (to T.K.) from Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT), and by grants from the JST‐PRESTO, AMED under Grant Number JP21gm5010002 and the Takeda Science Foundation (to T.K.). Funding Information: We thank R.L. Huganir, B.J. Knoll, and D.L. Turner for providing plasmids, and R.T. Yu for proofreading of the manuscript. We also thank the Core Instrumentation Facility, Keio University School of Medicine for help with Leica SP5 confocal microscopy. This work was supported by JSPS KAKENHI Grant Numbers JP26290015 and JP21H02655 (to T.K.) from Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT), and by grants from the JST-PRESTO, AMED under Grant Number JP21gm5010002 and the Takeda Science Foundation (to T.K.). Publisher Copyright: {\textcopyright} 2023 The Authors. Published under the terms of the CC BY 4.0 license.",
year = "2023",
month = mar,
day = "6",
doi = "10.15252/embr.202254701",
language = "English",
volume = "24",
journal = "EMBO Reports",
issn = "1469-221X",
publisher = "Nature Publishing Group",
number = "3",
}