Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain

Hiroshi Itoh, Kazuwa Nakao, Yoshihiko Saito, Takayuki Yamada, Gotaro Shirakami, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Shin ichi Suga, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Hiroo Imura

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 1989 Jan 16
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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