Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain

Hiroshi Itoh; Kazuwa Nakao; Yoshihiko Saito; Takayuki Yamada; Gotaro Shirakami; Masashi Mukoyama; Hiroshi Arai; Kiminori Hosoda; Shin ichi Suga; Naoto Minamino; Kenji Kangawa; Hisayuki Matsuo; Hiroo Imura

doi:10.1016/S0006-291X(89)80186-X

Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain

Hiroshi Itoh, Kazuwa Nakao, Yoshihiko Saito, Takayuki Yamada, Gotaro Shirakami, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Shin ichi Suga, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Hiroo Imura

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.

Original language	English
Pages (from-to)	120-128
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	158
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(89)80186-X
Publication status	Published - 1989 Jan 16
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(89)80186-X

Cite this

@article{aa443a34439b40e6ba9b1760946c55d0,

title = "Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain",

abstract = "We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.",

author = "Hiroshi Itoh and Kazuwa Nakao and Yoshihiko Saito and Takayuki Yamada and Gotaro Shirakami and Masashi Mukoyama and Hiroshi Arai and Kiminori Hosoda and Suga, {Shin ichi} and Naoto Minamino and Kenji Kangawa and Hisayuki Matsuo and Hiroo Imura",

note = "Funding Information: We thank Ms Hiroko Fumon and Ms Atsuko Furu for the excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare {"}Disorders of Adrenal Hormone{"} Research Committee, Japan, 1988, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-I and 63C-2) from the Japanese Ministry of Health and Welfare.",

year = "1989",

month = jan,

day = "16",

doi = "10.1016/S0006-291X(89)80186-X",

language = "English",

volume = "158",

pages = "120--128",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain

AU - Itoh, Hiroshi

AU - Nakao, Kazuwa

AU - Saito, Yoshihiko

AU - Yamada, Takayuki

AU - Shirakami, Gotaro

AU - Mukoyama, Masashi

AU - Arai, Hiroshi

AU - Hosoda, Kiminori

AU - Suga, Shin ichi

AU - Minamino, Naoto

AU - Kangawa, Kenji

AU - Matsuo, Hisayuki

AU - Imura, Hiroo

N1 - Funding Information: We thank Ms Hiroko Fumon and Ms Atsuko Furu for the excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1988, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-I and 63C-2) from the Japanese Ministry of Health and Welfare.

PY - 1989/1/16

Y1 - 1989/1/16

N2 - We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.

AB - We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.

UR - http://www.scopus.com/inward/record.url?scp=0024530855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024530855&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(89)80186-X

DO - 10.1016/S0006-291X(89)80186-X

M3 - Article

C2 - 2521447

AN - SCOPUS:0024530855

SN - 0006-291X

VL - 158

SP - 120

EP - 128

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this