TY - JOUR
T1 - Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors
AU - Goparaju, Sravan Kumar
AU - Kohda, Kazuhisa
AU - Ibata, Keiji
AU - Soma, Atsumi
AU - Nakatake, Yukhi
AU - Akiyama, Tomohiko
AU - Wakabayashi, Shunichi
AU - Matsushita, Misako
AU - Sakota, Miki
AU - Kimura, Hiromi
AU - Yuzaki, Michisuke
AU - Ko, Shigeru B.H.
AU - Ko, Minoru S.H.
N1 - Funding Information:
This work was in part supported by the Keio University Medical Science Fund-The Mitsunada Sakaguchi Laboratory, the CREST program from the Japan Science and Technology Agency (JST), and the Research Center Network for Realization of Regenerative Medicine, Japan Agency for Medical Research and Development (AMED).
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/2/13
Y1 - 2017/2/13
N2 - Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.
AB - Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.
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U2 - 10.1038/srep42367
DO - 10.1038/srep42367
M3 - Article
C2 - 28205555
AN - SCOPUS:85012296881
SN - 2045-2322
VL - 7
JO - Scientific reports
JF - Scientific reports
M1 - 42367
ER -
