TY - GEN
T1 - Rapid formation of size-controlled 3-dimensional hetero-spheroid using micro-rotational flow
AU - Kodama, T.
AU - Ota, H.
AU - Miki, N.
PY - 2011
Y1 - 2011
N2 - In this paper, we demonstrate formation of size-controlled 3-dimensional hetero-spheroids using a micro-fluidic device in approximately 2 min. The device collects cells in the center of a micro-chamber using micro-rotational flow as shown in Fig. 1. In previous work, we used this device to generate size-controlled HepG2 spheroids [1]. We applied it to form hetero-spheroids consisting of HepG2 and HUVEC. We experimentally found that the spheroid sizes could be controlled by the cell densities of the media independent of the ratio of HepG2 and HUVEC. Uniform dispersion of HepG2 and HUVEC in the formed spheroid was also experimentally verified.
AB - In this paper, we demonstrate formation of size-controlled 3-dimensional hetero-spheroids using a micro-fluidic device in approximately 2 min. The device collects cells in the center of a micro-chamber using micro-rotational flow as shown in Fig. 1. In previous work, we used this device to generate size-controlled HepG2 spheroids [1]. We applied it to form hetero-spheroids consisting of HepG2 and HUVEC. We experimentally found that the spheroid sizes could be controlled by the cell densities of the media independent of the ratio of HepG2 and HUVEC. Uniform dispersion of HepG2 and HUVEC in the formed spheroid was also experimentally verified.
KW - Bio MEMS
KW - Hetero-spheroid
KW - Micro-fluidics
KW - Micro-rotational flow
UR - http://www.scopus.com/inward/record.url?scp=84883749989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883749989&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883749989
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 359
EP - 361
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -