TY - JOUR
T1 - Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors
AU - Tanino, Mishie
AU - Sasajima, Toshio
AU - Nanjo, Hiroshi
AU - Akesaka, Shiori
AU - Kagaya, Masami
AU - Kimura, Taichi
AU - Ishida, Yusuke
AU - Oda, Masaya
AU - Takahashi, Masataka
AU - Sugawara, Taku
AU - Yoshioka, Toshiaki
AU - Nishihara, Hiroshi
AU - Akagami, Yoichi
AU - Goto, Akiteru
AU - Minamiya, Yoshihiro
AU - Tanaka, Shinya
N1 - Funding Information:
This work was supported in part by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (KAKENHI Grant Number 24590406) to M.T. and (KAKENHI Grant Number 23390311) to Y.M.
Publisher Copyright:
© 2014, The Japan Society of Brain Tumor Pathology.
PY - 2014/1
Y1 - 2014/1
N2 - Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
AB - Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
KW - Central nervous system-lymphoma (CNS-lymphoma)
KW - Glioma
KW - Rapid immunohistochemistry (R-IHC)
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U2 - 10.1007/s10014-014-0188-y
DO - 10.1007/s10014-014-0188-y
M3 - Article
C2 - 24807101
AN - SCOPUS:84939884180
SN - 1433-7398
VL - 32
SP - 12
EP - 19
JO - Brain tumor pathology
JF - Brain tumor pathology
IS - 1
ER -
