Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Mishie Tanino, Toshio Sasajima, Hiroshi Nanjo, Shiori Akesaka, Masami Kagaya, Taichi Kimura, Yusuke Ishida, Masaya Oda, Masataka Takahashi, Taku Sugawara, Toshiaki Yoshioka, Hiroshi Nishihara, Yoichi Akagami, Akiteru Goto, Yoshihiro Minamiya, Shinya Tanaka

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.

Original languageEnglish
Pages (from-to)12-19
Number of pages8
JournalBrain tumor pathology
Issue number1
Publication statusPublished - 2014 Jan
Externally publishedYes


  • Central nervous system-lymphoma (CNS-lymphoma)
  • Glioma
  • Rapid immunohistochemistry (R-IHC)

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research


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