Rapid quantification of the heteroplasmy of mutant mitochondrial DNAs in Leber's hereditary optic neuropathy using the Invader technology

Yukihiko Mashima, Makoto Nagano, Tomoyo Funayama, Qiang Zhang, Tohru Egashira, Jun Kudho, Nobuyoshi Shimizu, Yoshihisa Oguchi

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Purpose: To quantify the degree of heteroplasmy of a mitochondrial DNA (mtDNA) mutation in Leber's hereditary optic neuropathy (LHON) a biplex Invader® assay was applied. Methods: To determine the optimum condition for the Invader® assay, mtDNAs were assayed in various amounts of total DNA in 1-4-h incubations at 63°C. To evaluate the suitability of the Invader® assay to detect the three mutations, G3460A, G11778A, and T14484C, 10 ng of DNAs from 224 patients with bilateral optic atrophy was assayed. To quantify mtDNA heteroplasmy, a standard curve of known mixture ratios of mutation against calculation by the Invader® assay was constructed. Seventy-two of the 224 patients had one of the three mutations, which corresponded with the mutation detected earlier by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis. The percentages of mutant mtDNAs were calculated by the Invader® assay in five heteroplasmic families, including 30 individuals with the G11778A mutation. The results were compared with those calculated earlier by labeled polymerase chain reaction followed by single-strand conformation polymorphism (PCR-SSCP) analysis. Results: In 1-8 ng of DNA, the fluorescence intensity increased near linearly during a 4-h assay. With more than 16 ng of DNA, the intensities were saturated even at the 2-h assay. A linear relationship was observed between the results obtained from separate mixtures and from the Invader® assay analysis. Because two fluorescent intensities are not always the same, one of the two intensities was modified to adjust to that of the other. Complete concordance was observed between PCR-RFLP analysis and Invader® assay genotyping for the 224 patients. Results of percentage of heteroplasmy in five LHON families obtained by the Invader® assay were consistent with those by the PCR-SSCP analysis. Conclusions: Invader® assay is a simple, rapid, and reliable method of genotyping mtDNA mutations as well as quantifying heteroplasmy simultaneously under optimum conditions.

Original languageEnglish
Pages (from-to)268-276
Number of pages9
JournalClinical Biochemistry
Issue number4
Publication statusPublished - 2004 Apr


  • G11778A mutation
  • G3460A mutation
  • Heteroplasmy
  • Invader technology
  • Leber's hereditary optic neuropathy
  • Mitochondrial DNA
  • T14484CA mutation

ASJC Scopus subject areas

  • Clinical Biochemistry


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