Rat brain natriuretic peptide: Isolation from rat heart and tissue distribution

Kazuwa Nakao, Hiroshi Itoh, Yoshikazu Kambayashi, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin Ichi Suga, Michihisa Jougasaki, Yoshihiro Ogawa, Ken Inouye, Hiroo Imura

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


We have isolated a cardiac natriuretic peptide of 5, 000 d from atrial tissues from 500 rats and determined its amino acid sequence. The 5, 000 d atrial natriuretic factor was elucidated to be a 45 amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-K-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence (51-95) of the rat brain natriuretic peptide precursor deduced from the complementary DNA (cDNA) sequence. The cardiac natriuretic peptide with a molecular weight of 5, 000, or rat brain natriuretic peptide, was identified as the mqjor storage form and as the sole secretory form derived from the brain natriuretic peptide precursor in the rat heart. The rat brain natriuretic peptide level in the atrium was 3.68±0.61 μg/g, which represents about 4% of that of atrial natriuretic factor. Rat brain natriuretic peptide was also detected in the ventricle. The ratio of brain natriuretic peptide to atrial natriuretic peptide in the ventricle was approximately 30% and much higher than that in the atrium. Rat brain natriuretic peptide, however, was not detectable in the brain. We conclude that the 5, 000 d cardiac natriuretic peptide is rat brain natriuretic peptide with 45 amino acids derived from the brain natriuretic peptide precursor and is secreted from the rat heart as a novel cardiac hormone.

Original languageEnglish
Pages (from-to)774-778
Number of pages5
Issue number6
Publication statusPublished - 1990 Jun
Externally publishedYes


  • Atrial natriuretic peptide
  • Radioimmunoassay
  • Rat studies

ASJC Scopus subject areas

  • Internal Medicine


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