Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F-induced luteolysis in rats

Kazuhiro Minegishi, Mamoru Tanaka, Osamu Nishimura, Shinji Tanigaki, Kei Miyakoshi, Hitoshi Ishimoto, Yasunori Yoshimura

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


We investigated the contribution of neutrophils to prostaglandin (PG)F-induced luteolysis and the role of reactive oxygen species (ROS) as potential mediators of neutrophil accumulation in regressing corpora lutea of superovulated rats. On day 8 of pseudopregnancy, subcutaneous injection of PGF (500 μg) significantly increased rhodamine 6G-labeled leukocyte adhesion in luteal venules, as observed by intravital microscopy. Neutrophil accumulation was confirmed by significantly increased myeloperoxidase (MPO) activity. Pretreatment with anti-leukocyte antibody (CD18-directed monoclonal antibody, WT-3) significantly inhibited the PGF-induced increases in adherent leukocytes and MPO activity. Anti-leukocyte antibody also maintained serum progesterone concentrations. Pretreatment with oxygen free radical scavengers, superoxide dismutase (50,000 U/kg) and catalase (90,000 U/kg), also attenuated these PGF-induced alterations. Corpora lutea preloaded with dichlorodihydrofluorescein diacetate succinimidyl ester, a fluorescent indicator for determining intracellular ROS generation, exhibited an increase in fluorescence after PGF treatment. These findings suggest that leukocyte-endothelium interactions mediated by ROS generation are important in PGF-induced luteolysis in rats.

Original languageEnglish
Pages (from-to)E1308-E1315
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number6 46-6
Publication statusPublished - 2002 Dec 1


  • CD18 integrin
  • Corpus luteum
  • Intravital microscopy
  • Myeloperoxidase activity
  • Neutrophil

ASJC Scopus subject areas

  • General Medicine


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