TY - JOUR
T1 - Real-time measurement of nitric oxide using a bio-imaging and an electrochemical technique
AU - Kitamura, Yoshiichiro
AU - Ogawa, Hiroto
AU - Oka, Kotaro
N1 - Funding Information:
This study was partially performed with funding provided through Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government. We thank Dr D.W. Lübbers and Dr H. Baumgältl at the Max Planck Institute für molekulare Physiologie for teaching us the technique of fabricating the microcoaxial electrode. We also thank Dr K. Tanishita, Department of System Design Engineering, Faculty of Science and Technology, Keio University, Dr Y. Komai, Department of Bioengineering, The Whitaker Institute for Biomedical Engineering, University of California, San Diego, and Dr H. Sano, Institute of Biomedical Engineering, Faculty of Science and Technology, Keio University, and Dr T. Aomatsu, Department of Aerospace Engineering, Graduate School of Engineering, Nagoya University, Chikusa, for advice on proper use of the electrode.
PY - 2003/12/4
Y1 - 2003/12/4
N2 - Nitric oxide (NO) is an important mediator responsible for numerous physiological phenomena. Transient levels of NO in biological systems usually range from nanomolar to micromolar concentrations, with a rapid return to basal levels normally seen following these increases. Because NO can diffuse only over a local area in limited time due to such low levels of production and due to its short life-time prior to degradation, high spatial and temporal resolutions are required for direct and continuous NO measurement if the physiological role of NO is to be investigated in any system. For such purposes, analytical methods based on bio-imaging and electrochemical techniques for the measurement of NO are useful. In this paper, we describe the successful application of these methods to a number of biological systems. Specifically, complementary application of these methods demonstrate that it is possible to detect real-time NO production from nervous tissue with high spatial and temporal resolutions.
AB - Nitric oxide (NO) is an important mediator responsible for numerous physiological phenomena. Transient levels of NO in biological systems usually range from nanomolar to micromolar concentrations, with a rapid return to basal levels normally seen following these increases. Because NO can diffuse only over a local area in limited time due to such low levels of production and due to its short life-time prior to degradation, high spatial and temporal resolutions are required for direct and continuous NO measurement if the physiological role of NO is to be investigated in any system. For such purposes, analytical methods based on bio-imaging and electrochemical techniques for the measurement of NO are useful. In this paper, we describe the successful application of these methods to a number of biological systems. Specifically, complementary application of these methods demonstrate that it is possible to detect real-time NO production from nervous tissue with high spatial and temporal resolutions.
KW - Earthworm
KW - Endothelial cells
KW - NO-specific electrode
KW - NO-specific fluorescent dye
KW - Ventral nerve cord
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U2 - 10.1016/S0039-9140(03)00333-3
DO - 10.1016/S0039-9140(03)00333-3
M3 - Article
C2 - 18969236
AN - SCOPUS:0242665502
SN - 0039-9140
VL - 61
SP - 717
EP - 724
JO - Talanta
JF - Talanta
IS - 5
ER -