TY - JOUR
T1 - Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice
AU - Chung, David Y.
AU - Sugimoto, Kazutaka
AU - Fischer, Paul
AU - Böhm, Maximilian
AU - Takizawa, Tsubasa
AU - Sadeghian, Homa
AU - Morais, Andreia
AU - Harriott, Andrea
AU - Oka, Fumiaki
AU - Qin, Tao
AU - Henninger, Nils
AU - Yaseen, Mohammad A.
AU - Sakadžić, Sava
AU - Ayata, Cenk
N1 - Funding Information:
Funding was provided by the National Institute of Neurological Disorders and Stroke at the National Institutes of Health ( R25NS065743 to D.Y.C., R00AG042026 to M.A.Y., NS091499 to N.H., R01NS091230 and R01MH111359 to S.S., and P01NS055104 and R01NS102969 to C.A.); the American Heart Association ( 18POST34030369 to D.Y.C); the Brain Aneurysm Foundation’s Timothy P. Susco and Andrew David Heitman Foundation Chairs of Research to D.Y.C.; the Aneurysm and AVM Foundation to D.Y.C.; the International Headache Society to H.S.; the Japanese Heart Foundation and the Bayer Yakuhin Research Grant Abroad to F.O.; and Fondation Leducq , the Heitman Foundation , and the Ellison Foundation to C.A. We thank Saef Izzy for comments on the manuscript.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Background: Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. New method: We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. Results: OIS-WL detected 100% of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. Comparison with existing methods: : OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Conclusions: Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.
AB - Background: Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. New method: We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. Results: OIS-WL detected 100% of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. Comparison with existing methods: : OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Conclusions: Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.
KW - Cortical spreading depression
KW - Migraine
KW - Non-invasive
KW - Optical intrinsic signal imaging
KW - Stroke
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U2 - 10.1016/j.jneumeth.2018.09.001
DO - 10.1016/j.jneumeth.2018.09.001
M3 - Article
C2 - 30194041
AN - SCOPUS:85053054697
SN - 0165-0270
VL - 309
SP - 143
EP - 146
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
ER -