TY - JOUR
T1 - Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation
AU - Otani, Kei
AU - Niwa, Yuki
AU - Suzuki, Takehiro
AU - Sato, Natsumi
AU - Sasazawa, Yukiko
AU - Dohmae, Naoshi
AU - Simizu, Siro
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/4/6
Y1 - 2018/4/6
N2 - Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling.
AB - Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling.
KW - C-mannosylation
KW - Glycosylation
KW - Granulocyte colony-stimulating factor receptor (G-CSFR)
KW - Mass spectrometry
KW - Myeloid differentiation
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U2 - 10.1016/j.bbrc.2018.02.210
DO - 10.1016/j.bbrc.2018.02.210
M3 - Article
C2 - 29501745
AN - SCOPUS:85042904547
SN - 0006-291X
VL - 498
SP - 466
EP - 472
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -