Regulation of IL-2 production in Jurkat cells by Dictyostelium-derived factors

Katsunori Takahashi, Masami Murakami, Kohei Hosaka, Haruhisa Kikuchi, Yoshiteru Oshima, Yuzuru Kubohara

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Aims: Differentiation-inducing factors (DIFs) are chlorinated alkylphenones found in the cellular slime mold Dictyostelium discoideum. DIF derivatives exhibit antiproliferative activities and promote glucose consumption in mammalian cells in vitro. In this study, we assessed the ability of DIFs to regulate the immune system and investigated their mechanisms of action. Main methods: We examined the effects of 30 DIF derivatives on concanavalin A-induced IL-2 production (CIIP) in Jurkat T-cells. We also examined the effects of some DIF derivatives on the activity of AP-1 (activator protein-1), NFAT (nuclear factor of activated T-cells), and NFκB (nuclear factor kappa B), which are transcription factors required for CIIP. Key findings: Of the derivatives tested, some compounds suppressed CIIP as well as the known immunosuppressants cyclosporine A and FK506. A reporter gene assay revealed that 4 DIF derivatives tested suppressed CIIP, at least in part, by inhibiting the activity of AP-1, NFAT, and/or NFκB. Unlike cyclosporine A and FK506, the DIF derivatives had little effect on concanavalin A-induced interferon-γ production in Jurkat cells. Significance: The present results suggest that DIF derivatives could be developed as novel immunosuppressive drugs.

Original languageEnglish
Pages (from-to)438-443
Number of pages6
JournalLife Sciences
Issue number11-12
Publication statusPublished - 2009 Sept 9
Externally publishedYes


  • DIF
  • Dictyostelium
  • Differentiation-inducing factor
  • Immunosuppressive drug
  • Interferon-γ
  • Interleukin-2
  • Jurkat cells

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • Pharmacology, Toxicology and Pharmaceutics(all)


Dive into the research topics of 'Regulation of IL-2 production in Jurkat cells by Dictyostelium-derived factors'. Together they form a unique fingerprint.

Cite this