Regulation of mouse retinol dehydrogenases and retinal dehydrogenases in hepatocyte differentiation.

Kengo Tomita, Toshifumi Azuma, Sayaka Inokuchi, Naoto Kitamura, Takeshi Nishimura, Shinzo Kato, Hiromasa Ishii

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Retinoic acid (RA) is produced via two sequential reactions of retinol dehydrogenases (RDHs) and retinal dehydrogenases (RALDHs). We found that primary cultured mouse hepatocytes on a single collagen gel gradually lost hepatocyte specific morphology, and that another collagen gel overlay remarkably recovered it. The levels of albumin and liver-dominant expressed RDHs expression in hepatocytes paralleled their morphological change, decreased during single collagen gel culture, and were up-regulated by sequential collagen overlay. Quite similar to the expression changes, albumin and those RDHs' mRNA expression levels increased along with liver differentiation during pre- and postnatal liver development. Our data supports that all-trans and 9-cis RA, catalyzed by the RDHs, indeed play an important role in liver differentiation and regeneration.

Original languageEnglish
Pages (from-to)46-57
Number of pages12
JournalNihon Arukoru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence
Issue number1
Publication statusPublished - 2003 Feb
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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