TY - JOUR
T1 - Relationship of the aberrant DNA hypermethylation of cancer-related genes with carcinogenesis of endometrial cancer
AU - Banno, Kouji
AU - Yanokura, Megumi
AU - Susumu, Nobuyuki
AU - Kawaguchi, Makiko
AU - Hirao, Nobumaru
AU - Hirasawa, Akira
AU - Tsukazaki, Katsumi
AU - Aoki, Daisuke
PY - 2006/12
Y1 - 2006/12
N2 - Epigenetic abnormalities including the aberrant DNA hypermethylation of the promoter CpG islands play a key role in the mechanism of gene inactivation in cell carcinogenesis. To identify the genes associated with aberrant DNA hypermethylation in endometrial carcinogenesis, we studied the hypermethylation of the promoter regions of five genes: hMLH1, APC, E-cadherin, RAR-β and p16. The frequencies of aberrant hypermethylation were 40.4% (21/52) in hMLH1, 22% (11/50) in APC, 14% (7/50) in E- cadherin, and 2.3% (1/44) in RAR-β in endometrial cancer specimens. No aberrant DNA methylation was found in p16. In atypical endometrial hyperplasia, the frequencies of aberrant methylation were 14.3% (2/14) in hMLH1 and 7.3% (1/14) in APC, whereas normal endometrial cells showed no aberrant hypermethylation of any of the five genes. The high frequencies of the aberrant DNA hypermethylation of hMLH1 , APC and E-cadherin suggest that the methylation of the DNA mismatch repair and Wnt signal-related genes may be associated with endometrial carcinogenesis.
AB - Epigenetic abnormalities including the aberrant DNA hypermethylation of the promoter CpG islands play a key role in the mechanism of gene inactivation in cell carcinogenesis. To identify the genes associated with aberrant DNA hypermethylation in endometrial carcinogenesis, we studied the hypermethylation of the promoter regions of five genes: hMLH1, APC, E-cadherin, RAR-β and p16. The frequencies of aberrant hypermethylation were 40.4% (21/52) in hMLH1, 22% (11/50) in APC, 14% (7/50) in E- cadherin, and 2.3% (1/44) in RAR-β in endometrial cancer specimens. No aberrant DNA methylation was found in p16. In atypical endometrial hyperplasia, the frequencies of aberrant methylation were 14.3% (2/14) in hMLH1 and 7.3% (1/14) in APC, whereas normal endometrial cells showed no aberrant hypermethylation of any of the five genes. The high frequencies of the aberrant DNA hypermethylation of hMLH1 , APC and E-cadherin suggest that the methylation of the DNA mismatch repair and Wnt signal-related genes may be associated with endometrial carcinogenesis.
KW - Adenomatous polyposis coli
KW - DNA hypermethylation
KW - E-cadherin
KW - Endometrial cancer
KW - Human MutL homolog-1
KW - Retinoic acid receptor-β
UR - http://www.scopus.com/inward/record.url?scp=34548711004&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548711004&partnerID=8YFLogxK
U2 - 10.3892/or.16.6.1189
DO - 10.3892/or.16.6.1189
M3 - Article
C2 - 17089036
AN - SCOPUS:34548711004
SN - 1021-335X
VL - 16
SP - 1189
EP - 1196
JO - Oncology reports
JF - Oncology reports
IS - 6
ER -
