Repetitive DNA methylome analysis by small-scale and single-cell shotgun bisulfite sequencing

Hisato Kobayashi, Tasuku Koike, Akihiko Sakashita, Keisuke Tanaka, Soichiro Kumamoto, Tomohiro Kono

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Whole-genome shotgun bisulfite sequencing (WG-SBS) is currently the most powerful tool available for understanding genomewide cytosine methylation with single-base resolution; however, the high sequencing cost limits its widespread application, particularly for mammalian genomes. We mapped high- to low-coverage SBS short reads of mouse and human female developing germ cells to consensus sequences of repetitive elements that were multiplied in the respective host genome. This mapping strategy effectively identified active and evolutionarily young retrotransposon subfamilies and centromeric satellite repeats that were resistant to DNA demethylation during the investigated progressive stages of germ cell development. Notably, quantities of only tens of thousands of uniquely mapped reads provided sufficient sensitivity to allow for methylation analyses of multiple retrotransposons and satellite repeats in mice. Furthermore, we produced SBS results from single female murine germ cells by an improved multiplexing and amplification-free SBS method (scPBAT). The scPBAT results quantitatively provided ≥5× sequencing coverage for at least 30 repeats, and the individual methylation patterns detected were similar to the bulk cell-based results. Our single-cell methylome sequencing technique will allow researchers to investigate intergenic methylation characteristics from limited amounts of mammalian cells as well as cells from other organisms with genomic annotations.

Original languageEnglish
Pages (from-to)1209-1222
Number of pages14
JournalGenes to Cells
Issue number11
Publication statusPublished - 2016 Nov 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology


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