Reshuffling of the Bacillus subtilis 168 genome by multifold inversion

Azusa Kuroki; Tsutomu Toda; Kuniko Matsui; Rie Uotsu-Tomita; Masaru Tomita; Mitsuhiro Itaya

doi:10.1093/jb/mvm197

Reshuffling of the Bacillus subtilis 168 genome by multifold inversion

Azusa Kuroki, Tsutomu Toda, Kuniko Matsui, Rie Uotsu-Tomita, Masaru Tomita, Mitsuhiro Itaya

Faculty of Environment and Information Studies

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.

Original language	English
Pages (from-to)	97-105
Number of pages	9
Journal	Journal of biochemistry
Volume	143
Issue number	1
DOIs	https://doi.org/10.1093/jb/mvm197
Publication status	Published - 2008 Jan

Keywords

Antibiotic resistance
Competence
Homologous recombination
Inversion
Transformation
Translocation

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1093/jb/mvm197

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abstract = "The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.",

keywords = "Antibiotic resistance, Competence, Homologous recombination, Inversion, Transformation, Translocation",

author = "Azusa Kuroki and Tsutomu Toda and Kuniko Matsui and Rie Uotsu-Tomita and Masaru Tomita and Mitsuhiro Itaya",

note = "Funding Information: We thank to Drs K. Tsuge (Keio University, Yamagata, Japan) and Y. Naito (Keio University, Kanagawa, Japan) for useful discussions. This work was supported by Grant-in-Aid for the 21st Century Center of Excellence (COE) Program, entitled {\textquoteleft}{\textquoteleft}Understanding and Control of Life{\textquoteright}s Function via Systems Biology{\textquoteright}{\textquoteright} (to Keio University, Japan).",

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doi = "10.1093/jb/mvm197",

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AU - Kuroki, Azusa

AU - Toda, Tsutomu

AU - Matsui, Kuniko

AU - Uotsu-Tomita, Rie

AU - Tomita, Masaru

AU - Itaya, Mitsuhiro

N1 - Funding Information: We thank to Drs K. Tsuge (Keio University, Yamagata, Japan) and Y. Naito (Keio University, Kanagawa, Japan) for useful discussions. This work was supported by Grant-in-Aid for the 21st Century Center of Excellence (COE) Program, entitled ‘‘Understanding and Control of Life’s Function via Systems Biology’’ (to Keio University, Japan).

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N2 - The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.

AB - The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.

KW - Antibiotic resistance

KW - Competence

KW - Homologous recombination

KW - Inversion

KW - Transformation

KW - Translocation

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Reshuffling of the Bacillus subtilis 168 genome by multifold inversion

Abstract

Keywords

ASJC Scopus subject areas

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