Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168

Naoto Ohtani, Mitsuru Sato, Masaru Tomita, Mitsuhiro Itaya

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

Original languageEnglish
Pages (from-to)2472-2475
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number9
Publication statusPublished - 2008


  • Bacillus subtilis
  • BsuM
  • Conjugational transfer
  • Restriction modification

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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