Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168

Naoto Ohtani; Mitsuru Sato; Masaru Tomita; Mitsuhiro Itaya

doi:10.1271/bbb.80315

Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168

Naoto Ohtani, Mitsuru Sato, Masaru Tomita, Mitsuhiro Itaya

University

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

Original language	English
Pages (from-to)	2472-2475
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	9
DOIs	https://doi.org/10.1271/bbb.80315
Publication status	Published - 2008

Keywords

Bacillus subtilis
BsuM
Conjugational transfer
Restriction modification

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.80315

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abstract = "Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.",

keywords = "Bacillus subtilis, BsuM, Conjugational transfer, Restriction modification",

author = "Naoto Ohtani and Mitsuru Sato and Masaru Tomita and Mitsuhiro Itaya",

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AU - Ohtani, Naoto

AU - Sato, Mitsuru

AU - Tomita, Masaru

AU - Itaya, Mitsuhiro

PY - 2008

Y1 - 2008

N2 - Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

AB - Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

KW - Bacillus subtilis

KW - BsuM

KW - Conjugational transfer

KW - Restriction modification

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JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168

Abstract

Keywords

ASJC Scopus subject areas

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