Retrovirus-mediated gene transfer and expression cloning: Powerful tools in functional genomics

Toshio Kitamura, Yuko Koshino, Fumi Shibata, Toshihiko Oki, Hideaki Nakajima, Tetsuya Nosaka, Hidetoshi Kumagai

Research output: Contribution to journalReview articlepeer-review

613 Citations (Scopus)


Most of the human genome has now been sequenced and about 30,000 potential open reading frames have been identified, indicating that we use these 30,000 genes to functionally organize our biologic activities. However, functions of many genes are still unknown despite intensive efforts using bioinformatics as well as transgenic and knockout mice. Retrovirus-mediated gene transfer is a powerful tool that can be used to understand gene functions. We have developed a variety of retrovirus vectors and efficient packaging cell lines that have facilitated the development of efficient functional expression cloning methods. In this review, we describe retrovirus-mediated strategies used for investigation of gene functions and function-based screening strategies.

Original languageEnglish
Pages (from-to)1007-1014
Number of pages8
JournalExperimental Hematology
Issue number11
Publication statusPublished - 2003 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research


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