Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain

Masanobu Yamada; Keiji Shimizu; Yasuyoshi Miyao; Toru Hayakawa; Kazuhiro Ikenaka; Kensuke Nakahira; Kazunori Nakajima; Tetsushi Kagawa; Katsuhiko Mikoshiba

doi:10.1111/j.1349-7006.1992.tb02752.x

Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain

Masanobu Yamada, Keiji Shimizu, Yasuyoshi Miyao, Toru Hayakawa, Kazuhiro Ikenaka, Kensuke Nakahira, Kazunori Nakajima, Tetsushi Kagawa, Katsuhiko Mikoshiba

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coliβ‐galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV‐M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of β‐galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus‐mediated gene therapy for meningeal dissemination of malignant glioma.

Original language	English
Pages (from-to)	1244-1247
Number of pages	4
Journal	Japanese Journal of Cancer Research
Volume	83
Issue number	12
DOIs	https://doi.org/10.1111/j.1349-7006.1992.tb02752.x
Publication status	Published - 1992 Dec
Externally published	Yes

Keywords

Dissemination
Gene transfer
Glioma
Retroviral vector

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1349-7006.1992.tb02752.x

Cite this

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title = "Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain",

abstract = "A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coliβ‐galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV‐M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of β‐galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus‐mediated gene therapy for meningeal dissemination of malignant glioma.",

keywords = "Dissemination, Gene transfer, Glioma, Retroviral vector",

author = "Masanobu Yamada and Keiji Shimizu and Yasuyoshi Miyao and Toru Hayakawa and Kazuhiro Ikenaka and Kensuke Nakahira and Kazunori Nakajima and Tetsushi Kagawa and Katsuhiko Mikoshiba",

year = "1992",

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AU - Yamada, Masanobu

AU - Shimizu, Keiji

AU - Miyao, Yasuyoshi

AU - Hayakawa, Toru

AU - Ikenaka, Kazuhiro

AU - Nakahira, Kensuke

AU - Nakajima, Kazunori

AU - Kagawa, Tetsushi

AU - Mikoshiba, Katsuhiko

PY - 1992/12

Y1 - 1992/12

N2 - A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coliβ‐galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV‐M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of β‐galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus‐mediated gene therapy for meningeal dissemination of malignant glioma.

AB - A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coliβ‐galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV‐M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of β‐galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus‐mediated gene therapy for meningeal dissemination of malignant glioma.

KW - Dissemination

KW - Gene transfer

KW - Glioma

KW - Retroviral vector

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Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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