TY - JOUR
T1 - Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells
AU - Rho, M. C.
AU - Hayashi, M.
AU - Fukami, A.
AU - Obata, R.
AU - Sunazuka, T.
AU - Tomoda, H.
AU - Komiyama, K.
AU - Omura, S.
PY - 2000
Y1 - 2000
N2 - 7-O-Benzoylpyripyropene A (7-O-BzP), a semi-synthetic analog of pyripyropene, was investigated for its reversing effect on multidrug-resistant (MDR) tumor cells. 7-O-BzP (6.25 μg/ml) completely reversed resistance against vincristine and adriamycin in vincristine-resistant KB cells (VJ-300) and adriamycin-resistant P388 cells (P388/ADR), respectively. 7-O-BzP alone had no effect on the growth of drug sensitive and drug-resistant cells. 7-O-BzP (6.25 μg/ml) significantly enhanced accumulation of [3H]vincristine in VJ-300 cells and completely inhibited the binding of [3H]azidopine to the P-glycoprotein in VJ-300 cells and P388/ADR cells. The result suggests that 7-O-BzP effectively reverses P-glycoprotein-related MDR by interacting directly with P-glycoprotein in drug resistant VJ-300 and P388/ADR cells.
AB - 7-O-Benzoylpyripyropene A (7-O-BzP), a semi-synthetic analog of pyripyropene, was investigated for its reversing effect on multidrug-resistant (MDR) tumor cells. 7-O-BzP (6.25 μg/ml) completely reversed resistance against vincristine and adriamycin in vincristine-resistant KB cells (VJ-300) and adriamycin-resistant P388 cells (P388/ADR), respectively. 7-O-BzP alone had no effect on the growth of drug sensitive and drug-resistant cells. 7-O-BzP (6.25 μg/ml) significantly enhanced accumulation of [3H]vincristine in VJ-300 cells and completely inhibited the binding of [3H]azidopine to the P-glycoprotein in VJ-300 cells and P388/ADR cells. The result suggests that 7-O-BzP effectively reverses P-glycoprotein-related MDR by interacting directly with P-glycoprotein in drug resistant VJ-300 and P388/ADR cells.
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U2 - 10.7164/antibiotics.53.1201
DO - 10.7164/antibiotics.53.1201
M3 - Article
C2 - 11132967
AN - SCOPUS:0033762601
SN - 0021-8820
VL - 53
SP - 1201
EP - 1206
JO - Journal of Antibiotics
JF - Journal of Antibiotics
IS - 10
ER -