RNAi in transgenic animal models

Hidetoshi Hasuwa, Masaru Okabe

Research output: Chapter in Book/Report/Conference proceedingChapter


With completion of the “genome project” in human and mouse, we have entered the postgenome era, centered around the analysis of 30,000 to 40,000 gene functions. At present, ES cells and homologous recombination serve as powerful tools for many researchers aiming to elucidate the function of target genes in live animals. However, such methods are slow and cumbersome and are limited to mouse because of the technical difficulty in establishing ES cells in other species. Any improved technique should meet the following criteria: (a) simplicity of method to facilitate analysis of many genes in a short period of time, and (b) applicability to species other than mouse. RNAi could thus serve as an alternative, or supplemental, method to homologous re-combination.

Original languageEnglish
Title of host publicationGene Silencing by RNA Interference
Subtitle of host publicationTechnology and Application
PublisherCRC Press
Number of pages9
ISBN (Electronic)9780203489253
ISBN (Print)9780849321412
Publication statusPublished - 2004 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)


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