Role of oxygen-derived free radicals in fetal growth retardation induced by ischemia-reperfusion in rats

Hitoshi Ishimoto, Michiya Natori, Mamoru Tanaka, Toyohiko Miyazaki, Toshifumi Kobayashi, Yasunori Yoshimura

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43 Citations (Scopus)


We investigated the involvement of oxygen-derived free radicals in the pathogenesis of the intrauterine growth retardation (IUGR) induced in Sprague-Dawley rats by ischemia-reperfusion. On day 17 of gestation, rats received saline, superoxide dismutase (SOD, 50,000 U/kg), catalase (CAT, 50,000 U/kg), or SOD + CAT subcutaneously 1 h before induction of 30 min of ischemia of the right uterine horn. On day 21 the placental level of lipid peroxides was significantly increased (P < 0.001 vs. sham-operated group) and IUGR was induced (P < 0.001 vs. left horn) in the saline-treated group (n = 6). Pretreatment with SOD + CAT (n = 6) significantly inhibited the increase in placental lipid peroxides and prevented IUGR. The effect of ischemia- reperfusion on uterine blood flow, with or without pretreatment with radical scavengers, was investigated in separate experiments by laser-Doppler flowmetry. The induction of hypoperfusion 3 h after ischemia (blood flow 40 ± 5%, n = 6, P < 0.05) was blocked by pretreatment with SOD + CAT (n = 6). Results indicate that oxygen-derived free radicals may be important in the development of postischemic uteroplacental hypoperfusion and of ischemia- reperfusion-induced IUGR in the rat.

Original languageEnglish
Pages (from-to)H701-H705
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number2 41-2
Publication statusPublished - 1997 Feb
Externally publishedYes


  • intrauterine growth retardation
  • lipid peroxides
  • superoxide dismutase catalase
  • uterine blood flow

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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